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g2.drawLine(start, ypos, end, ypos);
g2.setComposite(origComposite);
}
/**
* Draw a translucent line
* @param g2
* @param start
* @param end
* @param ypos
*/
private void drawTranslucentJointedLine(Graphics2D g2, int start, int end, int ypos)
{
Composite origComposite = g2.getComposite();
g2.setComposite(translucent);
int mid = (int) ((end-start)/2.f)+start;
//g2.drawLine(start, ypos, end, ypos);
g2.drawLine(start, ypos, mid, ypos-5);
g2.drawLine(mid, ypos-5, end, ypos);
g2.setComposite(origComposite);
}
/**
* Display the SNPs for the given read.
* @param g2
* @param thisRead
* @param pixPerBase
* @param ypos
*/
private void showSNPsOnReads(Graphics2D g2, SAMRecord thisRead,
{
int thisStart = thisRead.getAlignmentStart();
int thisEnd = thisRead.getAlignmentEnd();
// use alignment blocks of the contiguous alignment of
// subsets of read bases to a reference sequence
List<AlignmentBlock> blocks = thisRead.getAlignmentBlocks();
new Range(thisStart+offset, thisEnd+offset), Bases.FORWARD);
offset = offset - getBaseAtStartOfView();
for(int i=0; i<blocks.size(); i++)
AlignmentBlock block = blocks.get(i);
for(int j=0; j<block.getLength(); j++)
int readPos = block.getReadStart()-1+j;
int refPos = block.getReferenceStart()+j;
if (Character.toUpperCase(refSeq[refPos-thisStart]) != readSeq[readPos])
{
g2.drawLine((int) ((refPos+offset) * pixPerBase), ypos + 2,
(int) ((refPos+offset) * pixPerBase), ypos - 2);
System.err.println(thisRead.getReadName()+" "+e.getMessage());
/**
* Add the alignment view to the supplied <code>JPanel</code> in
* a <code>JScrollPane</code>.
* @param mainPanel panel to add the alignment to
* @param autohide automatically hide the top panel containing the buttons
setDisplay(1, nbasesInView, null);
mainPanel.setLayout(new BorderLayout());
if(topPanel instanceof JPanel)
mainPanel.add(topPanel, BorderLayout.NORTH);
mainPanel.add(jspView, BorderLayout.CENTER);
JPanel bottomPanel = new JPanel(new BorderLayout());
coveragePanel = new CoveragePanel(this);
bottomPanel.add(coveragePanel, BorderLayout.CENTER);
//
snpPanel = new SnpPanel(this, bases);
bottomPanel.add(snpPanel, BorderLayout.NORTH);
if(feature_display == null)
{
scrollBar = new JScrollBar(JScrollBar.HORIZONTAL, 1, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.addAdjustmentListener(new AdjustmentListener()
{
public void adjustmentValueChanged(AdjustmentEvent e)
{
repaint();
if(isSNPplot)
snpPanel.repaint();
if(isCoverage)
coveragePanel.repaint();
}
});
bottomPanel.add(scrollBar, BorderLayout.SOUTH);
}
else
{
{
int seqLen = seqLengths.get((String) combo.getSelectedItem());
int artemisSeqLen = feature_display.getSequenceLength();
if(seqLen != artemisSeqLen)
{
int newIndex = -1;
for(int i=0; i<seqNames.size(); i++)
{
if(seqLengths.get(seqNames.get(i)) == artemisSeqLen)
{
// this looks like the correct sequence
combo.setSelectedIndex(i);
newIndex = i;
}
}
if(!Options.isBlackBeltMode())
{
String label[] = {
"The length of the sequence loaded does not match the length of",
"the default reference sequence in the BAM ("+seqNames.get(0)+").",
(newIndex == -1 ? "" : "The length does match the reference "+
};
new NonModalDialog(frame, label);
}
}
}
}
mainPanel.add(bottomPanel, BorderLayout.SOUTH);
coveragePanel.setPreferredSize(new Dimension(900, 100));
coveragePanel.setVisible(false);
snpPanel.setPreferredSize(new Dimension(900, 100));
snpPanel.setVisible(false);
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
private void addToViewMenu(final int thisBamIndex)
{
final File f = new File(bamList.get(thisBamIndex));
final JCheckBoxMenuItem cbBam = new JCheckBoxMenuItem(
f.getName(),
getImageIcon(getColourByCoverageColour(thisBamIndex)),
true);
bamFilesMenu.add(cbBam);
cbBam.addItemListener(new ItemListener() {
public void itemStateChanged(ItemEvent e) {
if(cbBam.isSelected())
hideBamList.remove(new Integer(thisBamIndex));
else
hideBamList.add(new Integer(thisBamIndex));
laststart = -1;
repaint();
}
});
}
/**
* Refresh the colour of the icons used to identify the
* BAM files.
*/
protected void refreshColourOfBamMenu()
{
final Component cs[] = bamFilesMenu.getMenuComponents();
for(Component c : cs)
{
if(c instanceof JCheckBoxMenuItem)
{
final JCheckBoxMenuItem cbBam = (JCheckBoxMenuItem) c;
final Color col = getColorByJCheckBoxMenuItem(cbBam);
if(col != null)
cbBam.setIcon(getImageIcon(col));
protected Color getColorByJCheckBoxMenuItem(JCheckBoxMenuItem cbBam)
{
final String bam = cbBam.getText();
for(int i=0; i<bamList.size(); i++)
{
final File f = new File(bamList.get(i));
if(f.getName().equals(bam))
return getColourByCoverageColour(i);
}
return null;
}
/**
* Create an icon of a box using the given colour.
* @param c
* @return
*/
{
BufferedImage image = (BufferedImage)this.createImage(10, 10);
Graphics2D g2 = image.createGraphics();
g2.setColor(c);
g2.fillRect(0, 0, 10, 10);
return new ImageIcon(image);
}
final JMenuItem addBam = new JMenuItem("Add BAM ...");
menu.add(addBam);
addBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
FileSelectionDialog bamFileSelection = new FileSelectionDialog(
null, false, "BamView", "BAM");
List<String> bamFiles = bamFileSelection.getFiles(".*\\.(bam|cram)$");
int count = bamList.size();
bamList.addAll(bamFileSelection.getFiles(".*\\.(bam|cram)$"));
for(int i=0; i<bamFiles.size(); i++)
addToViewMenu(i+count);
repaint();
}
});
bamFilesMenu.setFont(addBam.getFont());
final JMenuItem groupBams = new JMenuItem("Group BAMs ...");
final GroupBamFrame groupsFrame = new GroupBamFrame(this, bamFilesMenu);
groupBams.addActionListener(new ActionListener(){
public void actionPerformed(ActionEvent arg0)
{
groupsFrame.updateAndDisplay();
}
});
bamFilesMenu.add(groupBams);
bamFilesMenu.addSeparator();
menu.add(bamFilesMenu);
final JMenu analyse = new JMenu("Analyse");
menu.add(analyse);
final JMenuItem readCount = new JMenuItem("Read count of selected features ...");
if(feature_display == null)
readCount.setEnabled(false);
readCount.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
FeatureVector features = feature_display.getSelection().getAllFeatures();
JCheckBox overlap = new JCheckBox("Include all overlapping reads", true);
overlap.setToolTipText("Include reads that partially overlap the feature");
JCheckBox spliced = new JCheckBox("Introns included", true);
Box yBox = Box.createVerticalBox();
yBox.add(overlap);
yBox.add(spliced);
final ReadCountDialog opts = new ReadCountDialog(new JFrame(),
"Read Count Options", feature_display, yBox);
if(opts.getStatus() == -1)
return;
//JOptionPane.showMessageDialog(null, yBox, "Read Count Option", JOptionPane.INFORMATION_MESSAGE);
new MappedReads(features, (String)combo.getSelectedItem(), samFileReaderHash, bamList,
seqNames, offsetLengths, concatSequences, seqLengths,
samRecordFlagPredicate, samRecordMapQPredicate,
!overlap.isSelected(), spliced.isSelected());
}
});
final JMenuItem rpkm = new JMenuItem("RPKM value of selected features ...");
analyse.add(rpkm);
if(feature_display == null)
rpkm.setEnabled(false);
rpkm.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final FeatureVector features = feature_display.getSelection().getAllFeatures();
JCheckBox overlap = new JCheckBox("Include all overlapping reads", true);
overlap.setToolTipText("Include reads that partially overlap the feature");
JCheckBox spliced = new JCheckBox("Introns included", true);
JCheckBox allRefSeqs = new JCheckBox("Use reads mapped to all reference sequences", false);
Box yBox = Box.createVerticalBox();
yBox.add(overlap);
yBox.add(spliced);
if(seqLengths.size() > 1)
yBox.add(allRefSeqs);
final ReadCountDialog opts = new ReadCountDialog(new JFrame(),
"RPKM Options", feature_display, yBox);
if(opts.getStatus() == -1)
return;
int seqlen = 0;
if(feature_display != null)
seqlen = feature_display.getSequenceLength();
else if(bases != null)
seqlen = bases.getLength();
new MappedReads(features, (String)combo.getSelectedItem(),
samFileReaderHash, bamList, seqNames, offsetLengths, concatSequences,
seqLengths, seqlen, samRecordFlagPredicate, samRecordMapQPredicate,
!overlap.isSelected(), spliced.isSelected(), allRefSeqs.isSelected());
final JMenuItem createFeatures = new JMenuItem("Create features from coverage peaks ...");
analyse.add(createFeatures);
createFeatures.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(feature_display == null)
return;
new MappedReads(feature_display, (String)combo.getSelectedItem(),
samFileReaderHash, bamList, seqNames, offsetLengths,
concatSequences, seqLengths, samRecordFlagPredicate, samRecordMapQPredicate,
true);
}
});
for(int i=0; i<bamList.size(); i++)
addToViewMenu(i);
menu.add(new JSeparator());
cbStackView.setFont(viewMenu.getFont());
cbIsizeStackView.setFont(viewMenu.getFont());
cbPairedStackView.setFont(viewMenu.getFont());
cbStrandStackView.setFont(viewMenu.getFont());
cbCoverageView.setFont(viewMenu.getFont());
baseQualityColour.setFont(viewMenu.getFont());
colourByCoverageColour.setFont(viewMenu.getFont());
markInsertions.setFont(viewMenu.getFont());
cbIsizeStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
viewMenu.add(cbIsizeStackView);
cbStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
cbPairedStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
repaint();
}
});
viewMenu.add(cbPairedStackView);
cbStrandStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
viewMenu.add(cbStrandStackView);
cbCoverageView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
repaint();
}
});
viewMenu.add(cbCoverageView);
final JCheckBoxMenuItem checkBoxSNPs = new JCheckBoxMenuItem("SNP marks");
//
JMenu colourMenu = new JMenu("Colour By");
colourMenu.add(colourByCoverageColour);
baseQualityColour.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(baseQualityColour.isSelected())
{
checkBoxSNPs.setSelected(false);
isSNPs = false;
}
repaint();
}
});
colourMenu.add(baseQualityColour);
menu.add(colourMenu);
//
JCheckBoxMenuItem checkBoxOrientation = new JCheckBoxMenuItem("Orientation");
checkBoxOrientation.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isOrientation = !isOrientation;
repaint();
}
});
showMenu.add(checkBoxOrientation);
JCheckBoxMenuItem checkBoxSingle = new JCheckBoxMenuItem("Single Reads");
checkBoxSingle.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
repaint();
isSingle = !isSingle;
}
});
showMenu.add(checkBoxSingle);
checkBoxSNPs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (isSNPs && bases == null)
{
JOptionPane.showMessageDialog(null,
"No reference sequence supplied to identify SNPs.", "SNPs",
JOptionPane.INFORMATION_MESSAGE);
}
isSNPs = !isSNPs;
if(isSNPs)
baseQualityColour.setSelected(false);
repaint();
}
});
showMenu.add(checkBoxSNPs);
markInsertions.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
repaint();
}
});
showMenu.add(markInsertions);
JCheckBoxMenuItem checkBoxCoverage = new JCheckBoxMenuItem("Coverage");
checkBoxCoverage.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isCoverage = !isCoverage;
coveragePanel.setVisible(isCoverage);
graphMenu.add(checkBoxCoverage);
JCheckBoxMenuItem checkBoxSNP = new JCheckBoxMenuItem("SNP");
checkBoxSNP.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isSNPplot = !isSNPplot;
snpPanel.setVisible(isSNPplot);
repaint();
}
});
graphMenu.add(checkBoxSNP);
menu.add(graphMenu);
final JCheckBoxMenuItem checkBoxSync =
new JCheckBoxMenuItem("Asynchronous", asynchronous);
checkBoxSync.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
asynchronous = checkBoxSync.isSelected();
}
});
JMenu maxHeightMenu = new JMenu("BamView Height");
final String hgts[] =
{"500", "800", "1000", "1500", "2500", "5000", "50000"};
ButtonGroup bgroup = new ButtonGroup();
for(int i=0; i<hgts.length; i++)
{
final String hgt = hgts[i];
final JCheckBoxMenuItem maxHeightMenuItem = new JCheckBoxMenuItem(hgt);
bgroup.add(maxHeightMenuItem);
maxHeightMenuItem.setSelected(hgts[i].equals(Integer.toString(maxHeight)));
maxHeightMenu.add(maxHeightMenuItem);
maxHeightMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(maxHeightMenuItem.isSelected())
maxHeight = Integer.parseInt(hgt);
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
}
});
}
logMenuItem.setEnabled(isIsizeStackView());
logMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
logScale = logMenuItem.isSelected();
repaint();
}
});
JMenuItem filter = new JMenuItem("Filter Reads ...");
menu.add(filter);
filter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(filterFrame == null)
filterFrame = new SAMRecordFilter(BamView.this);
else
filterFrame.setVisible(true);
}
});
JMenuItem readList = new JMenuItem("List Reads ...");
menu.add(readList);
readList.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final JMenuItem bamSplitter = new JMenuItem("Clone window");
bamSplitter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView bamView = new BamView(new Vector<String>(bamList),
null, nbasesInView, entry_edit,
feature_display, bases, (JPanel) mainPanel.getParent(), null);
bamView.getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
if(feature_display != null)
{
feature_display.addDisplayAdjustmentListener(bamView);
feature_display.getSelection().addSelectionChangeListener(bamView);
}
}
});
menu.add(new JSeparator());
menu.add(bamSplitter);
//
JMenu coverageMenu = new JMenu("Coverage Options");
coverageView.init(this, 0.f, 0, 0);
coverageView.createMenus(coverageMenu);
viewMenu.add(new JSeparator());
viewMenu.add(coverageMenu);
private JComponent bamTopPanel(final JFrame frame)
{
final JComponent topPanel;
{
topPanel = new JPanel(new FlowLayout(FlowLayout.LEADING, 0, 0));
if(feature_display != null)
this.selection = feature_display.getSelection();
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
JMenu fileMenu = new JMenu("File");
topPanel.add(fileMenu);
JMenuItem readBam = new JMenuItem("Open new BamView ...");
fileMenu.add(readBam);
readBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
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BamView.main(s);
}
});
JMenuItem close = new JMenuItem("Close");
fileMenu.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView.this.setVisible(false);
Component comp = BamView.this;
while( !(comp instanceof JFrame) )
comp = comp.getParent();
((JFrame)comp).dispose();
}
});
JMenuItem exit = new JMenuItem("Exit");
fileMenu.add(new JSeparator());
fileMenu.add(exit);
exit.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
int status = JOptionPane.showConfirmDialog(BamView.this,
"Exit BamView?", "Exit",
JOptionPane.OK_CANCEL_OPTION);
if(status != JOptionPane.OK_OPTION)
return;
System.exit(0);
}
});
addKeyListener(new KeyAdapter()
{
public void keyPressed(final KeyEvent event)
{
switch (event.getKeyCode())
{
case KeyEvent.VK_UP:
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
break;
case KeyEvent.VK_DOWN:
if (showBaseAlignment)
break;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
break;
default:
break;
}
}
});
}
if(seqNames.size() > 1)
{
int len = 0;
for(int i=0; i<seqNames.size(); i++)
len += seqLengths.get(seqNames.get(i));
if(feature_display != null &&
len == feature_display.getSequenceLength())
concatSequences = true;
else if(bases != null &&
len == bases.getLength() )
concatSequences = true;
}
// auto hide top panel
final JCheckBox buttonAutoHide = new JCheckBox("Hide", (frame == null));
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buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
{
public void mouseDragged(MouseEvent event)
{
handleCanvasMouseDrag(event);
}
public void mouseMoved(MouseEvent e)
{
lastMousePoint = e.getPoint();
int thisHgt = HEIGHT;
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
if (y < thisHgt)
{
topPanel.setVisible(true);
}
else
{
if (buttonAutoHide.isSelected())
topPanel.setVisible(false);
}
mainPanel.repaint();
mainPanel.revalidate();
}
};
addMouseMotionListener(mouseMotionListener);
combo = new JComboBox(seqNames);
JTextComponent editor = (JTextComponent) combo.getEditor().getEditorComponent();
editor.setDocument(new AutoCompleteComboDocument(combo));
combo.setEditable(true);
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combo.setMaximumRowCount(20);
combo.addItemListener(new ItemListener()
{
public void itemStateChanged(ItemEvent e)
{
laststart = -1;
if(feature_display != null)
setZoomLevel(feature_display.getMaxVisibleBases());
else
setZoomLevel(BamView.this.nbasesInView);
}
});
topPanel.add(combo);
if(feature_display == null)
{
final JTextField baseText = new JTextField(8);
JButton goTo = new JButton("GoTo:");
goTo.setToolTipText("Go to base position");
goTo.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
try
{
int basePosition = Integer.parseInt(baseText.getText());
scrollBar.setValue(basePosition);
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(BamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
}
});
topPanel.add(goTo);
topPanel.add(baseText);
JButton zoomIn = new JButton("-");
zoomIn.setToolTipText("Zoom out (up arrow)");
Insets ins = new Insets(1,1,1,1);
zoomIn.setMargin(ins);
zoomIn.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
}
});
topPanel.add(zoomIn);
JButton zoomOut = new JButton("+");
zoomOut.setToolTipText("Zoom in (down arrow)");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
}
});
topPanel.add(zoomOut);
}
topPanel.add(buttonAutoHide);
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if(feature_display != null)
{
JButton close = new JButton("Close");
topPanel.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final JPanel containerPanel = (JPanel) mainPanel.getParent();
feature_display.removeDisplayAdjustmentListener(BamView.this);
feature_display.getSelection().removeSelectionChangeListener(BamView.this);
containerPanel.remove(mainPanel);
if(containerPanel.getComponentCount() > 0)
containerPanel.revalidate();
else
{
if(entry_edit != null)
entry_edit.setNGDivider();
else
containerPanel.setVisible(false);
}
}
});
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
private void setViewportMidPoint()
{
Point p = jspView.getViewport().getLocation();
p.y = (getHeight() - jspView.getViewport().getViewRect().height)/2;
jspView.getViewport().setViewPosition(p);
}
if(feature_display != null)
return feature_display.getForwardBaseAtLeftEdge();
else
return scrollBar.getValue();
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
if(isBaseAlignmentView(pixPerBase))
this.nbasesInView = (int)(mainPanel.getWidth()/pixPerBase);
if(ruler == null)
ruler = new Ruler();
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
markInsertions.setEnabled(true);
if(!cbCoverageView.isSelected() && nbasesInView >= MAX_BASES)
{
cbLastSelected = getSelectedCheckBoxMenuItem();
cbCoverageView.setSelected(true);
}
else if(cbCoverageView.isSelected() && nbasesInView < MAX_BASES && cbLastSelected != null)
{
cbLastSelected.setSelected(true);
cbLastSelected = null;
}
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
else
setViewportMidPoint();
markInsertions.setEnabled(false);
{
scrollBar.setValues(startValue, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.setBlockIncrement(nbasesInView);
}
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}