<SECT1 ID="CONCEPTS"> <TITLE>Concepts</TITLE> <PARA> Here are some general concepts about &prog; that may make the rest of this manual clearer. </PARA> <SECT2 ID="CONCEPTS-ENTRY"> <TITLE>The "Entry"</TITLE> <PARA> An "entry" in &prog;-speak is not necessarily a complete EMBL or GENBANK entry. In most places in this manual when we refer to an entry we mean a file that contains just the <ULINK URL="http://www.ebi.ac.uk/embl/Documentation/FT_definitions/feature_table.html">feature table</ULINK> lines (the <LITERAL>FT</LITERAL> lines) of an EMBL/GENBANK entry (see <XREF LINKEND="CONCEPTS-TABFILE">). After loading a sequence and opening an entry edit window (see <XREF LINKEND="LAUNCH-WINDOW-FILE-MENU-OPEN">) it is then possible to overlay many feature tables (see <XREF LINKEND="FILEMENU-READ-AN-ENTRY">). Each of these feature table files is called an entry by &prog; and it's features are kept separate from those of other entries. </PARA> <PARA> This meaning of the word "entry" is used by most of the items in the File menu (see <XREF LINKEND="FILEMENU">) and by the items in the Entry menu (see <XREF LINKEND="ENTRIESMENU">). </PARA> </SECT2> <SECT2 ID="CONCEPTS-FEATURE"> <TITLE>EMBL/Genbank Features</TITLE> <PARA> A "feature" in an EMBL or Genbank file is a region of DNA that has been annotated with a key/type (see <XREF LINKEND="CONCEPTS-KEY">) and zero or more qualifiers (see <XREF LINKEND="CONCEPTS-QUALIFIERS">). In an EMBL or Genbank formated file the features of a piece of DNA are listed in the feature table section (see <XREF LINKEND="CONCEPTS-TABFILE">). </PARA> </SECT2> <SECT2 ID="CONCEPTS-KEY"> <TITLE>EMBL/Genbank Feature Keys</TITLE> <PARA> All EMBL and Genbank features have exactly one "key" assigned to them. The key is the type of the feature. Examples include <LITERAL>CDS</LITERAL> (a <LITERAL>C</LITERAL>o<LITERAL>D</LITERAL>ing <LITERAL>S</LITERAL>equence), <LITERAL>intron</LITERAL> and <LITERAL>misc_feature</LITERAL> (<LITERAL>MISC</LITERAL>ellaneous <LITERAL>feature</LITERAL>). </PARA> <PARA> The <ULINK URL="http://www.ebi.ac.uk">EBI</ULINK> has a list of <ULINK URL="http://www.ebi.ac.uk/embl/Documentation/FT_definitions/feature_table.html">all possible feature keys</ULINK>. </PARA> </SECT2> <SECT2 ID="CONCEPTS-QUALIFIERS"> <TITLE>EMBL/Genbank Feature Qualifiers</TITLE> <PARA> The qualifiers of a feature in an EMBL or Genbank file are the notes and extra information about the feature. For example an <LITERAL>exon</LITERAL> feature might have a <LITERAL>/gene="ratC"</LITERAL> qualifier, meaning that the exon feature is part of a gene named <LITERAL>ratC</LITERAL>. </PARA> <PARA> The <ULINK URL="http://www.ebi.ac.uk">EBI</ULINK> has a list of <ULINK URL="http://www.ebi.ac.uk/embl/Documentation/FT_definitions/feature_table.html">all possible feature qualifiers</ULINK>. </PARA> </SECT2> <SECT2 ID="CONCEPTS-TABFILE"> <TITLE>"Tab" Files or "Table" Files</TITLE> <PARA> An EMBL or Genbank file that only contains a feature table (just <LITERAL>FT</LITERAL> lines, no sequence or header lines) is called a "table" file, or sometimes just a "tab" file because the often has a name like "somefile.tab". </PARA> </SECT2> <SECT2 ID="CONCEPTS-ACTIVEENTRY"> <TITLE>The Active Entries</TITLE> <PARA> All entries in &prog; are considered to be "active" or "inactive". The overview, DNA view and feature list parts of the main window will only display features from active entries. To find out how to set the active and inactive entries see <XREF LINKEND="ENTRIESMENU">. </PARA> </SECT2> <SECT2 ID="CONCEPTS-DEFAULTENTRY"> <TITLE>The Default Entry</TITLE> <PARA> Many actions (such as creating features) require an entry to be identified as the source or destination for the action. Some actions, such as "Move Selected Features To ..." in the edit menu, will explicitly ask for an entry, but some assume that the action refers to a "default entry" that was previously set by the user. </PARA> <PARA> The default entry can be set by using the "Set Default Entry ..." menu item in the Entries menu (see <XREF LINKEND="ENTRIESMENU-SET-DEFAULT-ENTRY">)<![ %artemis-only; [ or by using the entry buttons (see <XREF LINKEND="ENTRYBUTTONS">)]]>. </PARA> </SECT2> <SECT2 ID="CONCEPTS-SEGMENT"> <TITLE>Feature Segments</TITLE> <PARA> The term "segment" in the context of a CDS feature means "exon". We use the term "segment", because non-CDS features (such as misc_feature) can have exon-like parts too, but the term "exon" would be inappropriate in that case. </PARA> </SECT2> <SECT2 ID="CONCEPTS-SELECTION"> <TITLE>The Selection</TITLE> <PARA> In common with applications like word processors and graphics programs, &prog; allows the user to "select" the objects that the program will act on. The objects to act on in &prog; are features, feature segments or bases. If a feature segment is added to the selection, the feature that contains the segment is implicitly added as well. The current selection can be changed with the Select Menu (see <XREF LINKEND="SELECTMENU">) or using the mouse (see <XREF LINKEND="VIEWS-SELECTION"><![ %artemis-only; [ and <XREF LINKEND="FEATURELIST-SELECTION">]]>). </PARA> </SECT2> <SECT2 ID="CONCEPTS-COLOUR"> <TITLE>Feature Colours</TITLE> <PARA> Each feature displayed in &prog; can be given a colour. The available colours are set in the options file (see <XREF LINKEND="OPTIONS-CHAPT">) and are assigned to a feature by adding a <LITERAL>/colour</LITERAL> qualifier (see <XREF LINKEND="EDITMENU-EDIT-SELECTED-FEATURES">). Currently there are two ways of specifying feature colours. The first way uses a single number. For example red is colour 2, so adding <LITERAL>/colour=2</LITERAL> as a feature qualifier will make that feature red. The second way is to specify the red, green and blue components of the colour. Each of the components can take values from 0 to 255, with 255 being the most intense. For example <LITERAL>/colour=255 0 0</LITERAL> is another way to give a feature the colour red. If no <LITERAL>/colour</LITERAL> qualifier is set for a feature a default colour is used (the default colours are also specified in the options file). </PARA> </SECT2> </SECT1>