/* BamView * * created: 2012 * * This file is part of Artemis * * Copyright(C) 2012 Genome Research Limited * * This program is free software; you can redistribute it and/or * modify it under the terms of the GNU General Public License * as published by the Free Software Foundation; either version 2 * of the License, or(at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with this program; if not, write to the Free Software * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. * */ package uk.ac.sanger.artemis.components.alignment; import net.sf.picard.reference.ReferenceSequence; import net.sf.picard.reference.ReferenceSequenceFile; import net.sf.samtools.SAMSequenceDictionary; import uk.ac.sanger.artemis.Entry; import uk.ac.sanger.artemis.io.Range; import uk.ac.sanger.artemis.sequence.Bases; import uk.ac.sanger.artemis.util.OutOfRangeException; class CRAMReferenceSequenceFile implements ReferenceSequenceFile { private Entry sequence; private BamView bamView; CRAMReferenceSequenceFile(final Entry sequence, final BamView bamView) { this.sequence = sequence; this.bamView = bamView; } /** * Retrieves the complete sequence described by this contig. * @param contig contig whose data should be returned. * @return The full sequence associated with this contig. * @throws UnsupportedOperationException if !sIndexed. */ public ReferenceSequence getSequence(String contig) { return getSubsequenceAt(contig, 1L, sequence.getBases().getLength()); //return new ReferenceSequence(sequence.getName(), 0, // sequence.getBases().getForwardStrand().getStrandBases().getBytes()); } public SAMSequenceDictionary getSequenceDictionary() { return null; } /** * Gets the subsequence of the contig in the range [start,stop] * @param contig Contig whose subsequence to retrieve. * @param start inclusive, 1-based start of region. * @param stop inclusive, 1-based stop of region. * @return The partial reference sequence associated with this range. * @throws UnsupportedOperationException if !sIndexed. */ public ReferenceSequence getSubsequenceAt(String contig, long start, long stop ) { try { if(bamView.isConcatSequences()) { int offset = bamView.getSequenceOffset(contig); start += offset; stop += offset; } return new ReferenceSequence(sequence.getName(), 0, sequence.getBases().getSubSequence(new Range((int)start, (int)stop), Bases.FORWARD).getBytes()); } catch (OutOfRangeException e) { e.printStackTrace(); } return null; } /** * @return true if getSequence and getSubsequenceAt methods are allowed. */ public boolean isIndexed() { return true; } public ReferenceSequence nextSequence() { return null; } public void reset() { } public void close() { } }