BamView.java

      {
        handleCanvasMouseDrag(event);
      }
      
      public void mouseMoved(MouseEvent e)
      {
        lastMousePoint = e.getPoint();
        
        int thisHgt = HEIGHT;
        if (thisHgt < 5)
          thisHgt = 15;

        int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
        if (y < thisHgt)
        {
          topPanel.setVisible(true);
        }
        else
        {
          if (buttonAutoHide.isSelected())
            topPanel.setVisible(false);
        }
        mainPanel.repaint();
        mainPanel.revalidate();
      }
    };
    addMouseMotionListener(mouseMotionListener);

    
    combo = new SequenceComboBox(seqNames){
      private static final long serialVersionUID = 1L;
      public void update(IndexReferenceEvent event)
      {
        laststart = -1;
        if(feature_display != null)
          setZoomLevel(feature_display.getMaxVisibleBases());
        else
          setZoomLevel(BamView.this.nbasesInView);
      }
    };
    topPanel.add(combo);

    if(feature_display == null)
    {
      final JTextField baseText = new JTextField(8);
      JButton goTo = new JButton("GoTo:");
      goTo.setToolTipText("Go to base position");
      goTo.addActionListener(new ActionListener()
      {
        public void actionPerformed(ActionEvent e)
        {
          try
          {
            int basePosition = Integer.parseInt(baseText.getText());
            scrollBar.setValue(basePosition);
          }
          catch (NumberFormatException nfe)
          {
            JOptionPane.showMessageDialog(BamView.this,
                "Expecting a base number!", "Number Format",
                JOptionPane.WARNING_MESSAGE);
          }
        }
      });
      topPanel.add(goTo);
      topPanel.add(baseText);

      JButton zoomIn = new JButton("-");
      zoomIn.setToolTipText("Zoom out (up arrow)");
      Insets ins = new Insets(1,1,1,1);
      zoomIn.setMargin(ins);
      zoomIn.addActionListener(new ActionListener()
      {
        public void actionPerformed(ActionEvent e)
        {
          setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
        }
      });
      topPanel.add(zoomIn);

      JButton zoomOut = new JButton("+");
      zoomOut.setToolTipText("Zoom in (down arrow)");
      zoomOut.setMargin(ins);
      zoomOut.addActionListener(new ActionListener()
      {
        public void actionPerformed(ActionEvent e)
        {
          if (showBaseAlignment)
            return;
          setZoomLevel((int) (BamView.this.nbasesInView * .9));
        }
      });
      topPanel.add(zoomOut);
    }
    
    topPanel.add(buttonAutoHide);
    
    if(feature_display != null)
    {
      JButton close = new JButton("Close");
      topPanel.add(close);
      close.addActionListener(new ActionListener()
      {
        public void actionPerformed(ActionEvent e)
        {
          final JPanel containerPanel = (JPanel) mainPanel.getParent();
          feature_display.removeDisplayAdjustmentListener(BamView.this);
          feature_display.getSelection().removeSelectionChangeListener(BamView.this);
          containerPanel.remove(mainPanel);
          
          if(containerPanel.getComponentCount() > 0)
            containerPanel.revalidate();
          else
          {
            if(entry_edit != null)
              entry_edit.setNGDivider();
            else
              containerPanel.setVisible(false);
          }
        }
      });
    }
    return topPanel;
  }
  
  public void setVisible(boolean visible)
  {
    super.setVisible(visible);
    mainPanel.setVisible(visible);
  }
  
  private void setViewportMidPoint()
  {
    Point p = jspView.getViewport().getLocation();
    p.y = (getHeight() - jspView.getViewport().getViewRect().height)/2;
    jspView.getViewport().setViewPosition(p);
  }
  
  private void setViewportBtm()
  {
    jspView.getVerticalScrollBar().setValue(
        jspView.getVerticalScrollBar().getMaximum());
  }
  
  protected int getBaseAtStartOfView()
  {
    if(feature_display != null)
      return feature_display.getForwardBaseAtLeftEdge();
    else
      return scrollBar.getValue();
  }
  
  /**
   * Set the panel size based on the number of bases visible
   * and repaint.
   * @param nbasesInView
   */
  private void setZoomLevel(final int nbasesInView)
  {
    int startValue = getBaseAtStartOfView();
    this.nbasesInView = nbasesInView;
    float pixPerBase = getPixPerBaseByWidth(); 

    if(isBaseAlignmentView(pixPerBase))
    {
      pixPerBase = ALIGNMENT_PIX_PER_BASE;
      this.nbasesInView = (int)(mainPanel.getWidth()/pixPerBase);
      jspView.getVerticalScrollBar().setValue(0);
      
      if(ruler == null)
        ruler = new Ruler();
      jspView.setColumnHeaderView(ruler);
      showBaseAlignment = true;
      baseQualityColour.setEnabled(true);
      markInsertions.setEnabled(true);
    }
    else if(jspView != null)
    {
      if(!isCoverageView(pixPerBase) && nbasesInView >= MAX_BASES)
      {
        cbLastSelected = getSelectedCheckBoxMenuItem();
        cbCoverageView.setSelected(true);
        coverageView.setPlotByStrand(false);
      }
      else if(isCoverageView(pixPerBase) && nbasesInView < MAX_BASES && cbLastSelected != null)
      {
        cbLastSelected.setSelected(true);
        cbLastSelected = null;
      }
      
      jspView.setColumnHeaderView(null);
      
      if(!isStrandStackView())
        setViewportBtm();
      else
        setViewportMidPoint();
      showBaseAlignment = false;
      baseQualityColour.setEnabled(false);
      markInsertions.setEnabled(false);
    }
    
    if(scrollBar != null)
    {
      scrollBar.setValues(startValue, nbasesInView, 1, 
             getMaxBasesInPanel(getSequenceLength()));
      scrollBar.setUnitIncrement(nbasesInView/20);
      scrollBar.setBlockIncrement(nbasesInView);
    }
  }

  
  /**
   * Set the start and end base positions to display.
   * @param start
   * @param end
   * @param event
   */
  public void setDisplay(int start,
                         int end,
                         DisplayAdjustmentEvent event)
  {
    this.startBase = start;
    this.endBase   = end;
    this.nbasesInView = end-start+1;
    lastMousePoint = null;

    float pixPerBase;
    if(jspView.getViewport().getViewRect().width > 0)
      pixPerBase = getPixPerBaseByWidth();
    else
    {
      if(feature_display == null)
        pixPerBase = 1000.f/(float)(end-start+1);
      else
        pixPerBase = feature_display.getWidth()/(float)(end-start+1);
    }
    
    Dimension d = new Dimension();
    d.setSize(nbasesInView*pixPerBase, maxHeight);
    setPreferredSize(d);
    
    if(event == null)
    {
      this.startBase = -1;
      this.endBase   = -1;
    }
  }
  
  /**
   * Return an Artemis entry from a file 
   * @param entryFileName
   * @param entryGroup
   * @return
   * @throws NoSequenceException
   */
  private Entry getEntry(final String entryFileName, final EntryGroup entryGroup) 
                   throws NoSequenceException
  {
    final Document entry_document = DocumentFactory.makeDocument(entryFileName);
    final EntryInformation artemis_entry_information =
      Options.getArtemisEntryInformation();
    
    //System.out.println(entryFileName);
    final uk.ac.sanger.artemis.io.Entry new_embl_entry =
      EntryFileDialog.getEntryFromFile(null, entry_document,
                                       artemis_entry_information,
                                       false);

    if(new_embl_entry == null)  // the read failed
      return null;

    Entry entry = null;
    try
    {
      if(entryGroup.getSequenceEntry() != null)
        bases = entryGroup.getSequenceEntry().getBases();
      
      if(bases == null)
      {
        entry = new Entry(new_embl_entry);
        bases = entry.getBases();
      }
      else
        entry = new Entry(bases,new_embl_entry);
      
      entryGroup.add(entry);
    } 
    catch(OutOfRangeException e) 
    {
      new MessageDialog(null, "read failed: one of the features in " +
          entryFileName + " has an out of range " +
                        "location: " + e.getMessage());
    }
    return entry;
  }
  
  private boolean isShowScale()
  {
    return (feature_display == null ? true : false);
  }

  public JScrollPane getJspView()
  {
    return jspView;
  }
  
  /**
   *  Handle a mouse drag event on the drawing canvas.
   **/
  private void handleCanvasMouseDrag(final MouseEvent event)
  {
    if(event.getButton() == MouseEvent.BUTTON3 || bases == null) 
      return;
    
    highlightSAMRecord = null;
    if(event.getClickCount() > 1)
    {
      getSelection().clear();
      repaint();
      return;  
    }
    
    highlightRange(event, 
        MouseEvent.BUTTON1_DOWN_MASK & MouseEvent.BUTTON2_DOWN_MASK);
  }
  
  /**
   * 
   * @param event
   * @param onmask
   */
  protected void highlightRange(MouseEvent event, int onmask)
  {
    int seqLength = getSequenceLength();
    float pixPerBase = getPixPerBaseByWidth();
    int start = (int) ( ( (event.getPoint().getX())/pixPerBase) + getBaseAtStartOfView() );
    
    if(start < 1)
      start = 1;
    if(start > seqLength)
      start = seqLength;
    
    if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
      dragStart = start;
    else if((event.getModifiersEx() & onmask) != onmask)
      dragStart = -1;
    
    MarkerRange drag_range;
    try
    {
      if(dragStart < 0)
        drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
      else
        drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
      getSelection().setMarkerRange(drag_range);
      repaint();
    }
    catch (OutOfRangeException e)
    {
      e.printStackTrace();
    }
  }
  
  /**
   * Get the colour for the given read given to it by the coverage plot.
   * @param samRecord
   * @return
   */
  private Color getColourByCoverageColour(BamViewRecord samRecord)
  {
    short fileIndex = 0;
    if(bamList.size()>1)
      fileIndex = samRecord.bamIndex;
    return getColourByCoverageColour(fileIndex); 
  }
  
  private Color getColourByCoverageColour(final short fileIndex)
  {
    LineAttributes lines[] = CoveragePanel.getLineAttributes(bamList.size());
    return lines[fileIndex].getLineColour(); 
  }
  

  protected int getMaxBases()
  {
    return MAX_BASES;
  }
  
  protected void setMaxBases(int max)
  {
    MAX_BASES = max;
  }
  
  private boolean isStackView()
  {
    return cbStackView.isSelected();  
  }
  
  private boolean isPairedStackView()
  {
    return cbPairedStackView.isSelected();
  }
  
  private boolean isStrandStackView()
  {
    return cbStrandStackView.isSelected();
  }
  
  private boolean isCoverageView(float pixPerBase)
  {
    if(isBaseAlignmentView(pixPerBase))
      return false;
    return cbCoverageView.isSelected() || cbCoverageStrandView.isSelected() || cbCoverageHeatMap.isSelected();
  }
  
  private boolean isIsizeStackView()
  {
    return cbIsizeStackView.isSelected();
  }
  
  private boolean isBaseAlignmentView(float pixPerBase)
  {
    if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
      return true;
    return false;
  }
  
  private JCheckBoxMenuItem getSelectedCheckBoxMenuItem()
  {
    if(isStackView())
      return cbStackView;
    if(isPairedStackView())
      return cbPairedStackView;
    if(isStrandStackView())
      return cbStrandStackView;
    if(isIsizeStackView())
      return cbIsizeStackView;
    if(cbCoverageView.isSelected())
      return cbCoverageView;
    if(cbCoverageHeatMap.isSelected())
      return cbCoverageHeatMap;
    return cbCoverageStrandView;
  }
  
  protected Selection getSelection()
  {
    return selection;
  }
  
  protected List<BamViewRecord> getReadsInView()
  {
    return readsInView;
  }
  
  protected int getBasesInView()
  {
    return nbasesInView;
  }
  
  protected void setHighlightSAMRecord(BamViewRecord highlightSAMRecord)
  {
    this.highlightSAMRecord = highlightSAMRecord;
  }
  
  protected BamViewRecord getHighlightSAMRecord()
  {
    return highlightSAMRecord;
  }
  
  protected FeatureDisplay getFeatureDisplay()
  {
    return feature_display;
  }
  
  /**
   * @return the combo
   */
  public SequenceComboBox getCombo()
  {
    return combo;
  }
  
  private String getVersion()
  {
    final ClassLoader cl = this.getClass().getClassLoader();
    try
    {
      String line;
      InputStream in = cl.getResourceAsStream("etc/versions");
      BufferedReader reader = new BufferedReader(new InputStreamReader(in));
      while((line = reader.readLine()) != null)
      {
        if(line.startsWith("BamView"))
          return line.substring( "BamView".length() ).trim();
      }
      reader.close();
      in.close();
    }
    catch (Exception ex)
    {
    }
    return null;
  }
  
  /**
   * Open another BamView window
   */
  public void openBamView(final List<String> bamsList)
  {
    BamView bamView = new BamView(bamsList, 
        null, nbasesInView, entry_edit,
        feature_display, bases, (JPanel) mainPanel.getParent(), null);
    bamView.getJspView().getVerticalScrollBar().setValue(
        bamView.getJspView().getVerticalScrollBar().getMaximum());
    getJspView().getVerticalScrollBar().setValue(
        bamView.getJspView().getVerticalScrollBar().getMaximum());

    int start = getBaseAtStartOfView();
    setDisplay(start, nbasesInView+start, null);
    if(feature_display != null)
    {
      feature_display.addDisplayAdjustmentListener(bamView);
      feature_display.getSelection().addSelectionChangeListener(bamView);
    }
  }
  
  /**
   * Artemis event notification
   */
  public void displayAdjustmentValueChanged(final DisplayAdjustmentEvent event)
  {
    if(event.getType() == DisplayAdjustmentEvent.REV_COMP_EVENT &&
       event.isRevCompDisplay())
      JOptionPane.showMessageDialog(this, 
          "Flipping the display is not supported by BamView.", "Warning", 
          JOptionPane.WARNING_MESSAGE);

    if(!asynchronous)
    {
      // if not asynchronous
      displayAdjustmentWork(event);
      return;
    }
    
    SwingWorker worker = new SwingWorker()
    {
      public Object construct()
      {
        try
        {
          Thread.sleep(500);
        }
        catch (InterruptedException e)
        {
          e.printStackTrace();
        }
        
        if(event.getStart() != ((FeatureDisplay)event.getSource()).getForwardBaseAtLeftEdge())
        {
          waitingFrame.showWaiting("waiting...", mainPanel);
          return null;
        }
      
        displayAdjustmentWork(event);
        waitingFrame.setVisible(false);
        return null;
      }
    };
    worker.start();
  }
  
  /**
   * Carry out the display agjustment event action.
   * @param event
   */
  private void displayAdjustmentWork(final DisplayAdjustmentEvent event)
  {
    if(event.getType() == DisplayAdjustmentEvent.SCALE_ADJUST_EVENT)
    {
      laststart = -1;

      BamView.this.startBase = event.getStart();
      BamView.this.endBase   = event.getEnd();

      int width = feature_display.getMaxVisibleBases();
      setZoomLevel(width);
      repaint();
    }
    else
    {
      setDisplay(event.getStart(), 
        event.getStart()+feature_display.getMaxVisibleBases(), event);
      repaint();
    }
  }
  
  public void selectionChanged(SelectionChangeEvent event)
  {
    repaint();
  }
  
  private class Ruler extends JPanel
  {
    private static final long serialVersionUID = 1L;
    protected int start;
    protected int end;
    protected String refSeq;

    public Ruler()
    {
      super();
      setPreferredSize(new Dimension(mainPanel.getWidth(), 26));
      setBackground(Color.white);
    }

    public void paintComponent(Graphics g)
    {
      super.paintComponent(g);
      Graphics2D g2 = (Graphics2D)g;
      drawBaseScale(g2, start, end, 12);
    }

    private void drawBaseScale(Graphics2D g2, int start, int end, int ypos)
    {
      int startMark = (((int)(start/10))*10)+1;
      if(end > getSequenceLength())
        end = getSequenceLength();

      for(int i=startMark; i<end; i+=20)
      {
        int xpos = (i-start)*ALIGNMENT_PIX_PER_BASE;
        g2.drawString(Integer.toString(i), xpos, ypos);
      }
        
      for(int i=startMark; i<end; i+=10)
      {
        int xpos = (i-start)*ALIGNMENT_PIX_PER_BASE;
        xpos+=(ALIGNMENT_PIX_PER_BASE/2);
        g2.drawLine(xpos, ypos+1, xpos, ypos+5);
      }
      
      if(refSeq != null)
      {
        ypos+=15;
        g2.setColor(LIGHT_GREY);
        g2.fillRect(0, ypos-11, getWidth(), 11);

        g2.translate(0, 16);
        drawSelectionRange(g2, ALIGNMENT_PIX_PER_BASE, start, end, Color.yellow);
        g2.translate(0, -16);
        g2.setColor(Color.black);
        g2.drawString(refSeq, 0, ypos-2);
      }
    }
  }
  
  /**
  * Popup menu listener
  */
  class PopupListener extends MouseAdapter
  {
	private JMenuItem gotoMateMenuItem;
	private JMenuItem showDetails;
	private JMenu coverageMenu;
	private JMenuItem createGroup;
	
    public void mouseClicked(MouseEvent e)
    {
      if(e.isPopupTrigger() ||
         e.getButton() == MouseEvent.BUTTON3)
        return;
      
      BamView.this.requestFocus();
      
      if(e.getClickCount() > 1)
        getSelection().clear(); 
      else if(e.getButton() == MouseEvent.BUTTON1)
      {
        if(isCoverageView(getPixPerBaseByWidth()))
          coverageView.singleClick(e.isShiftDown(),
              e.getPoint().y-getJspView().getViewport().getViewPosition().y);
        else
          highlightSAMRecord = mouseOverSAMRecord;
      }
      else
        highlightRange(e, MouseEvent.BUTTON2_DOWN_MASK);
      repaint();
    }
    
    public void mousePressed(MouseEvent e)
    {
      maybeShowPopup(e);
    }

    public void mouseReleased(MouseEvent e)
    {
      dragStart = -1;
      maybeShowPopup(e);
    }

    private void maybeShowPopup(MouseEvent e)
    {
      if(e.isPopupTrigger())
      {       
        //
        // main menu options
        if(popup == null)
        {
          popup = new JPopupMenu();
          createMenus(popup);
        }

        //
        // coverage heatmap menu options
        if(coverageMenu != null)
          popup.remove(coverageMenu);
        if(isCoverageView(getPixPerBaseByWidth()) && coverageView.isPlotHeatMap())
        {
          if(coverageMenu == null)
          {
            coverageMenu = new JMenu("Coverage HeatMap");
            coverageView.createMenus(coverageMenu);
            
            final JCheckBoxMenuItem coverageGrid = new JCheckBoxMenuItem("Show heatmap grid", false);
            coverageGrid.addActionListener(new ActionListener()
            {
              public void actionPerformed(ActionEvent e) 
              {
                coverageView.showLabels(coverageGrid.isSelected());
              }
            });
            coverageMenu.add(coverageGrid);

            createGroup = new JMenuItem("Create group from selected BAMs");
            createGroup.addActionListener(new ActionListener()
            {
              private int n = 1;
              public void actionPerformed(ActionEvent e) 
              {
                String groupName = "group_"+n;
                groupsFrame.addGroup(groupName);
                final List<String> selected = coverageView.getSelected();
                for(String sel: selected)
                  groupsFrame.addToGroup((new File(sel)).getName(), groupName);
                groupsFrame.updateAndDisplay();
                n++;
              }
            });
            coverageMenu.add(createGroup);
          }
          createGroup.setEnabled(coverageView.hasSelectedBams());
          popup.add(coverageMenu);
        }

        if(gotoMateMenuItem != null)
          popup.remove(gotoMateMenuItem);
        if(showDetails != null)
          popup.remove(showDetails);

        if( mouseOverSAMRecord != null && 
            mouseOverSAMRecord.sam.getReadPairedFlag() &&
           !mouseOverSAMRecord.sam.getMateUnmappedFlag() )
        {
          final BamViewRecord thisSAMRecord = mouseOverSAMRecord;
          gotoMateMenuItem = new JMenuItem("Go to mate of : "+
              thisSAMRecord.sam.getReadName());
          gotoMateMenuItem.addActionListener(new ActionListener()
          {
			public void actionPerformed(ActionEvent e) 
			{
			  String name = thisSAMRecord.sam.getMateReferenceName();
			  if(name.equals("="))
			    name = thisSAMRecord.sam.getReferenceName();
			  int offset = getSequenceOffset(name);
			  if(feature_display != null)
			    feature_display.makeBaseVisible(
			        thisSAMRecord.sam.getMateAlignmentStart()+offset);
			  else
			    scrollBar.setValue(
			        thisSAMRecord.sam.getMateAlignmentStart()+offset-
			        (nbasesInView/2));
			  
			  highlightSAMRecord = thisSAMRecord; 
			}  
          });
          popup.add(gotoMateMenuItem);
        }  
          
        if( mouseOverSAMRecord != null)
        {
          final BamViewRecord thisSAMRecord = mouseOverSAMRecord;
          showDetails = new JMenuItem("Show details of : "+
              thisSAMRecord.sam.getReadName());
          showDetails.addActionListener(new ActionListener()
          {
            public void actionPerformed(ActionEvent e) 
            {
              openFileViewer(thisSAMRecord.sam, getMate(thisSAMRecord), bamList);
            }
          });
          popup.add(showDetails);
        }
        popup.show(e.getComponent(),
                e.getX(), e.getY());
      }
    }
  }
  
  protected static void openFileViewer(SAMRecord readRecord, SAMRecord mateRecord, List<String> bamList)
  {
    FileViewer viewDetail = new FileViewer(readRecord.getReadName(), true, false, false);
    appendToDetailView(readRecord, mateRecord, viewDetail, bamList);
  }
  
  private static void appendToDetailView(final SAMRecord sam, 
                                         final SAMRecord mate, 
                                         final FileViewer viewer, 
                                         final List<String> bamList)
  {
    if(bamList.size() > 1 && sam.getAttribute("FL") != null)
    {
      int bamIdx = (Integer)sam.getAttribute("FL");
      if(bamIdx < bamList.size())
        viewer.appendString("File                  "+bamList.get(bamIdx)+"\n\n", Level.INFO);
    }
    
    viewer.appendString("Read Name             "+sam.getReadName()+"\n", Level.INFO);
    viewer.appendString("Coordinates           "+sam.getAlignmentStart()+".."+
                                                 sam.getAlignmentEnd()+"\n", Level.DEBUG);
    viewer.appendString("Length                "+sam.getReadLength()+"\n", Level.DEBUG);
    viewer.appendString("Reference Name        "+sam.getReferenceName()+"\n", Level.DEBUG);
    viewer.appendString("Inferred Size         "+sam.getInferredInsertSize()+"\n", Level.DEBUG);
    viewer.appendString("Mapping Quality       "+sam.getMappingQuality()+"\n", Level.DEBUG);
    viewer.appendString("Cigar String          "+sam.getCigarString()+"\n", Level.DEBUG);
    viewer.appendString("Strand                "+
        (sam.getReadNegativeStrandFlag() ? "-\n\n" : "+\n\n"), Level.DEBUG);
    
    if(sam.getReadPairedFlag())
    {     
      if(mate != null)
      {
        viewer.appendString("Mate Coordinates      "+mate.getAlignmentStart()+".."+
                                                     mate.getAlignmentEnd()+"\n", Level.DEBUG);
        viewer.appendString("Mate Length           "+mate.getReadLength()+"\n", Level.DEBUG);
        viewer.appendString("Mate Reference Name   "+mate.getReferenceName()+"\n", Level.DEBUG);
        viewer.appendString("Mate Inferred Size    "+mate.getInferredInsertSize()+"\n", Level.DEBUG);
        viewer.appendString("Mate Mapping Quality  "+mate.getMappingQuality()+"\n", Level.DEBUG);
        viewer.appendString("Mate Cigar String     "+mate.getCigarString()+"\n", Level.DEBUG);
      }
      else
      {
        viewer.appendString("Mate Start Coordinate "+sam.getMateAlignmentStart()+"\n", Level.DEBUG);
        viewer.appendString("Mate Reference Name   "+sam.getMateReferenceName()+"\n", Level.DEBUG);
      }
      viewer.appendString("Mate Strand           "+
          (sam.getMateNegativeStrandFlag() ? "-" : "+"), Level.DEBUG);
    }

    viewer.appendString("\n\nFlags:", Level.INFO);
    viewer.appendString("\nDuplicate Read    "+
        (sam.getDuplicateReadFlag() ? "yes" : "no"), Level.DEBUG);
    
    viewer.appendString("\nRead Paired       "+
        (sam.getReadPairedFlag() ? "yes" : "no"), Level.DEBUG);
    if(sam.getReadPairedFlag())
    {
      viewer.appendString("\nFirst of Pair     "+
        (sam.getFirstOfPairFlag() ? "yes" : "no"), Level.DEBUG);
      viewer.appendString("\nMate Unmapped     "+
        (sam.getMateUnmappedFlag() ? "yes" : "no"), Level.DEBUG);  
      viewer.appendString("\nProper Pair       "+
        (sam.getProperPairFlag() ? "yes" : "no"), Level.DEBUG);
    }
    viewer.appendString("\nRead Fails Vendor\nQuality Check     "+
        (sam.getReadFailsVendorQualityCheckFlag() ? "yes" : "no"), Level.DEBUG);
    viewer.appendString("\nRead Unmapped     "+
        (sam.getReadUnmappedFlag() ? "yes" : "no"), Level.DEBUG);
    
    if(sam.getReadPairedFlag())
      viewer.appendString("\nSecond Of Pair    "+
        (sam.getSecondOfPairFlag() ? "yes" : "no"), Level.DEBUG);
    
    viewer.appendString("\n\nRead Bases:\n", Level.INFO);
    wrapReadBases(sam, viewer);
    
    if(sam.getReadPairedFlag() && mate != null)
    {
      viewer.appendString("\nMate Read Bases:\n", Level.INFO);
      wrapReadBases(mate, viewer);
    }
  }
  
  private static void wrapReadBases(final SAMRecord sam, 
                             final FileViewer viewer)
  {
    final String seq = new String(sam.getReadBases());
    final int MAX_SEQ_LINE_LENGTH = 100;
    for(int i=0; i<=seq.length(); i+=MAX_SEQ_LINE_LENGTH)
    {
      int iend = i+MAX_SEQ_LINE_LENGTH;
      if(iend > seq.length())
        iend = seq.length();
      viewer.appendString(seq.substring(i, iend)+"\n", Level.DEBUG);
    }
  }

  /**
   * Query for the mate of a read
   * @param mate
   * @return
   */
  protected SAMRecord getMate(BamViewRecord thisSAMRecord)
  {
    if(!thisSAMRecord.sam.getReadPairedFlag())  // read is not paired in sequencing
      return null;
    
    SAMRecord mate = null;
    try
    {
      short fileIndex = 0;
      if(bamList.size()>1 && thisSAMRecord.bamIndex > 0)
        fileIndex = thisSAMRecord.bamIndex;
      String bam = bamList.get(fileIndex);  
      final SAMFileReader inputSam = getSAMFileReader(bam);
      mate = inputSam.queryMate(thisSAMRecord.sam);
    }
    catch (Exception e)
    {
      logger4j.warn("WARN: BamView.getMate() :: "+e.getMessage());
      e.printStackTrace();
    }
    return mate;
  }
  
  protected SAMRecordPredicate getSamRecordFlagPredicate()
  {
    return samRecordFlagPredicate;
  }

  protected void setSamRecordFlagPredicate(
      SAMRecordPredicate samRecordFlagPredicate)
  {
    laststart = -1;
    lastend = -1;
    this.samRecordFlagPredicate = samRecordFlagPredicate;
  }
  
  protected SAMRecordMapQPredicate getSamRecordMapQPredicate()
  {
    return samRecordMapQPredicate;
  }

  protected void setSamRecordMapQPredicate(
      SAMRecordMapQPredicate samRecordMapQPredicate)
  {
    laststart = -1;
    lastend = -1;
    this.samRecordMapQPredicate = samRecordMapQPredicate;
  }
  
  /**
   * @return the concatSequences
   */
  protected boolean isConcatSequences()
  {
    return concatSequences;
  }

  class PairedRead
  {
    BamViewRecord sam1;
    BamViewRecord sam2;
  }
  
  class CreateFeatures
  {
    CreateFeatures(final GroupBamFrame groupsFrame)
    {
      final TextFieldInt threshold = new TextFieldInt();
      final TextFieldInt minSize = new TextFieldInt();
      final TextFieldInt minBams = new TextFieldInt();
      
      threshold.setValue(6);
      minSize.setValue(6);
      minBams.setValue( (groupsFrame.getNumberOfGroups() == 1 ?
          bamList.size() : groupsFrame.getMaximumBamsInGroup()) );
      
      final JPanel gridPanel = new JPanel(new GridBagLayout());
      GridBagConstraints c = new GridBagConstraints();
      c.anchor = GridBagConstraints.WEST;
      c.fill = GridBagConstraints.HORIZONTAL;
      c.gridx = 0;
      c.gridy = 0;
      
      gridPanel.add(new JLabel("Minimum number of reads:"), c);
      c.gridy++;
      gridPanel.add(threshold, c);
      
      c.gridy++;
      gridPanel.add(new JSeparator(), c);