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private void addToViewMenu(final int thisBamIndex)
{
File f = new File(bamList.get(thisBamIndex));
final JCheckBoxMenuItem cbBam = new JCheckBoxMenuItem(
f.getName(), true);
bamFilesMenu.add(cbBam);
cbBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(cbBam.isSelected())
hideBamList.remove(new Integer(thisBamIndex));
else
hideBamList.add(new Integer(thisBamIndex));
laststart = -1;
repaint();
}
});
}
final JMenuItem addBam = new JMenuItem("Add BAM ...");
menu.add(addBam);
addBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
FileSelectionDialog bamFileSelection = new FileSelectionDialog(
null, false, "BamView", "BAM");
List<String> bamFiles = bamFileSelection.getFiles(".*\\.bam$");
int count = bamList.size();
bamList.addAll(bamFileSelection.getFiles(".*\\.bam$"));
for(int i=0; i<bamFiles.size(); i++)
addToViewMenu(i+count);
repaint();
}
});
bamFilesMenu.setFont(addBam.getFont());
menu.add(bamFilesMenu);
final JMenu analyse = new JMenu("Analyse");
menu.add(analyse);
final JMenuItem readCount = new JMenuItem("Read count of selected features ...");
if(feature_display == null)
readCount.setEnabled(false);
readCount.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
FeatureVector features = feature_display.getSelection().getAllFeatures();
JCheckBox overlap = new JCheckBox("Include all overlapping reads", true);
overlap.setToolTipText("Include reads that partially overlap the feature");
JCheckBox spliced = new JCheckBox("Introns included", true);
Box yBox = Box.createVerticalBox();
yBox.add(overlap);
yBox.add(spliced);
JOptionPane.showMessageDialog(null, yBox, "Read Count Option", JOptionPane.INFORMATION_MESSAGE);
new MappedReads(features, (String)combo.getSelectedItem(), samFileReaderHash, bamList,
seqNames, offsetLengths, concatSequences, seqLengths,
samRecordFlagPredicate, samRecordMapQPredicate,
!overlap.isSelected(), spliced.isSelected());
}
});
final JMenuItem rpkm = new JMenuItem("RPKM value of selected features ...");
analyse.add(rpkm);
if(feature_display == null)
rpkm.setEnabled(false);
rpkm.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
FeatureVector features = feature_display.getSelection().getAllFeatures();
JCheckBox overlap = new JCheckBox("Include all overlapping reads", true);
overlap.setToolTipText("Include reads that partially overlap the feature");
JCheckBox spliced = new JCheckBox("Introns included", true);
JCheckBox allRefSeqs = new JCheckBox("Use reads mapped to all reference sequences", false);
Box yBox = Box.createVerticalBox();
yBox.add(overlap);
yBox.add(spliced);
if(seqLengths.size() > 1)
yBox.add(allRefSeqs);
JOptionPane.showMessageDialog(null, yBox, "Read Count Option", JOptionPane.INFORMATION_MESSAGE);
int seqlen = 0;
if(feature_display != null)
seqlen = feature_display.getSequenceLength();
else if(bases != null)
seqlen = bases.getLength();
new MappedReads(features, (String)combo.getSelectedItem(),
samFileReaderHash, bamList, seqNames, offsetLengths, concatSequences,
seqLengths, seqlen, samRecordFlagPredicate, samRecordMapQPredicate,
!overlap.isSelected(), spliced.isSelected(), allRefSeqs.isSelected());
for(int i=0; i<bamList.size(); i++)
addToViewMenu(i);
menu.add(new JSeparator());
cbStackView.setFont(viewMenu.getFont());
cbIsizeStackView.setFont(viewMenu.getFont());
cbPairedStackView.setFont(viewMenu.getFont());
cbStrandStackView.setFont(viewMenu.getFont());
cbCoverageView.setFont(viewMenu.getFont());
baseQualityColour.setFont(viewMenu.getFont());
colourByCoverageColour.setFont(viewMenu.getFont());
markInsertions.setFont(viewMenu.getFont());
cbIsizeStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
viewMenu.add(cbIsizeStackView);
cbStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
cbPairedStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
repaint();
}
});
viewMenu.add(cbPairedStackView);
cbStrandStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
viewMenu.add(cbStrandStackView);
cbCoverageView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
logMenuItem.setEnabled(isIsizeStackView());
repaint();
}
});
viewMenu.add(cbCoverageView);
final JCheckBoxMenuItem checkBoxSNPs = new JCheckBoxMenuItem("SNP marks");
//
JMenu colourMenu = new JMenu("Colour By");
colourMenu.add(colourByCoverageColour);
baseQualityColour.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(baseQualityColour.isSelected())
{
checkBoxSNPs.setSelected(false);
isSNPs = false;
}
repaint();
}
});
colourMenu.add(baseQualityColour);
menu.add(colourMenu);
//
JCheckBoxMenuItem checkBoxOrientation = new JCheckBoxMenuItem("Orientation");
checkBoxOrientation.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isOrientation = !isOrientation;
repaint();
}
});
showMenu.add(checkBoxOrientation);
JCheckBoxMenuItem checkBoxSingle = new JCheckBoxMenuItem("Single Reads");
checkBoxSingle.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
repaint();
isSingle = !isSingle;
}
});
showMenu.add(checkBoxSingle);
checkBoxSNPs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (isSNPs && bases == null)
{
JOptionPane.showMessageDialog(null,
"No reference sequence supplied to identify SNPs.", "SNPs",
JOptionPane.INFORMATION_MESSAGE);
}
isSNPs = !isSNPs;
if(isSNPs)
baseQualityColour.setSelected(false);
repaint();
}
});
showMenu.add(checkBoxSNPs);
markInsertions.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
repaint();
}
});
showMenu.add(markInsertions);
JCheckBoxMenuItem checkBoxCoverage = new JCheckBoxMenuItem("Coverage");
checkBoxCoverage.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isCoverage = !isCoverage;
coveragePanel.setVisible(isCoverage);
graphMenu.add(checkBoxCoverage);
JCheckBoxMenuItem checkBoxSNP = new JCheckBoxMenuItem("SNP");
checkBoxSNP.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isSNPplot = !isSNPplot;
snpPanel.setVisible(isSNPplot);
repaint();
}
});
graphMenu.add(checkBoxSNP);
menu.add(graphMenu);
final JCheckBoxMenuItem checkBoxSync =
new JCheckBoxMenuItem("Asynchronous", asynchronous);
checkBoxSync.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
asynchronous = checkBoxSync.isSelected();
}
});
JMenu maxHeightMenu = new JMenu("BamView Height");
final String hgts[] =
{"500", "800", "1000", "1500", "2500", "5000", "50000"};
ButtonGroup bgroup = new ButtonGroup();
for(int i=0; i<hgts.length; i++)
{
final String hgt = hgts[i];
final JCheckBoxMenuItem maxHeightMenuItem = new JCheckBoxMenuItem(hgt);
bgroup.add(maxHeightMenuItem);
maxHeightMenuItem.setSelected(hgts[i].equals(Integer.toString(maxHeight)));
maxHeightMenu.add(maxHeightMenuItem);
maxHeightMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(maxHeightMenuItem.isSelected())
maxHeight = Integer.parseInt(hgt);
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
}
});
}
logMenuItem.setEnabled(isIsizeStackView());
logMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
logScale = logMenuItem.isSelected();
repaint();
}
});
JMenuItem filter = new JMenuItem("Filter Reads ...");
menu.add(filter);
filter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(filterFrame == null)
filterFrame = new SAMRecordFilter(BamView.this);
else
filterFrame.setVisible(true);
}
});
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final JMenuItem bamSplitter = new JMenuItem("Clone window");
bamSplitter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView bamView = new BamView(bamList, null, nbasesInView,
feature_display, bases, (JPanel) mainPanel.getParent(), null);
bamView.getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
if(feature_display != null)
{
feature_display.addDisplayAdjustmentListener(bamView);
feature_display.getSelection().addSelectionChangeListener(bamView);
}
}
});
menu.add(new JSeparator());
menu.add(bamSplitter);
//
JMenu coverageMenu = new JMenu("Coverage Options");
coverageView.init(this, 0.f, 0, 0);
coverageView.createMenus(coverageMenu);
viewMenu.add(new JSeparator());
viewMenu.add(coverageMenu);
private JComponent bamTopPanel(final JFrame frame)
{
final JComponent topPanel;
if(feature_display != null)
this.selection = feature_display.getSelection();
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topPanel = new JPanel(new FlowLayout(FlowLayout.LEADING, 0, 0));
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
JMenu fileMenu = new JMenu("File");
topPanel.add(fileMenu);
JMenuItem readBam = new JMenuItem("Open new BamView ...");
fileMenu.add(readBam);
readBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
String[] s = new String[0];
BamView.main(s);
}
});
JMenuItem close = new JMenuItem("Close");
fileMenu.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView.this.setVisible(false);
Component comp = BamView.this;
while( !(comp instanceof JFrame) )
comp = comp.getParent();
((JFrame)comp).dispose();
}
});
JMenuItem exit = new JMenuItem("Exit");
fileMenu.add(new JSeparator());
fileMenu.add(exit);
exit.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
int status = JOptionPane.showConfirmDialog(BamView.this,
"Exit BamView?", "Exit",
JOptionPane.OK_CANCEL_OPTION);
if(status != JOptionPane.OK_OPTION)
return;
System.exit(0);
}
});
addKeyListener(new KeyAdapter()
{
public void keyPressed(final KeyEvent event)
{
switch (event.getKeyCode())
{
case KeyEvent.VK_UP:
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
break;
case KeyEvent.VK_DOWN:
if (showBaseAlignment)
break;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
break;
default:
break;
}
}
});
}
if(seqNames.size() > 1)
{
int len = 0;
for(int i=0; i<seqNames.size(); i++)
len += seqLengths.get(seqNames.get(i));
if(feature_display != null &&
len == feature_display.getSequenceLength())
concatSequences = true;
else if(bases != null &&
len == bases.getLength() )
concatSequences = true;
}
// auto hide top panel
final JCheckBox buttonAutoHide = new JCheckBox("Hide", (frame == null));
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buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
{
public void mouseDragged(MouseEvent event)
{
handleCanvasMouseDrag(event);
}
public void mouseMoved(MouseEvent e)
{
lastMousePoint = e.getPoint();
int thisHgt = HEIGHT;
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
if (y < thisHgt)
{
topPanel.setVisible(true);
}
else
{
if (buttonAutoHide.isSelected())
topPanel.setVisible(false);
}
mainPanel.repaint();
mainPanel.revalidate();
}
};
addMouseMotionListener(mouseMotionListener);
combo = new JComboBox(seqNames);
JTextComponent editor = (JTextComponent) combo.getEditor().getEditorComponent();
editor.setDocument(new AutoCompleteComboDocument(combo));
combo.setEditable(true);
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combo.setMaximumRowCount(20);
combo.addItemListener(new ItemListener()
{
public void itemStateChanged(ItemEvent e)
{
laststart = -1;
if(feature_display != null)
setZoomLevel(feature_display.getMaxVisibleBases());
else
setZoomLevel(BamView.this.nbasesInView);
}
});
topPanel.add(combo);
if(feature_display == null)
{
final JTextField baseText = new JTextField(8);
JButton goTo = new JButton("GoTo:");
goTo.setToolTipText("Go to base position");
goTo.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
try
{
int basePosition = Integer.parseInt(baseText.getText());
scrollBar.setValue(basePosition);
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(BamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
}
});
topPanel.add(goTo);
topPanel.add(baseText);
JButton zoomIn = new JButton("-");
Insets ins = new Insets(1,1,1,1);
zoomIn.setMargin(ins);
zoomIn.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
}
});
topPanel.add(zoomIn);
JButton zoomOut = new JButton("+");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
}
});
topPanel.add(zoomOut);
}
topPanel.add(buttonAutoHide);
return topPanel;
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
private void setViewportMidPoint()
{
Point p = jspView.getViewport().getLocation();
p.y = (getHeight() - jspView.getViewport().getViewRect().height)/2;
jspView.getViewport().setViewPosition(p);
}
if(feature_display != null)
return feature_display.getForwardBaseAtLeftEdge();
else
return scrollBar.getValue();
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
if(isBaseAlignmentView(pixPerBase))
this.nbasesInView = (int)(mainPanel.getWidth()/pixPerBase);
if(ruler == null)
ruler = new Ruler();
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
markInsertions.setEnabled(true);
if(!cbCoverageView.isSelected() && nbasesInView >= MAX_BASES)
{
cbLastSelected = getSelectedCheckBoxMenuItem();
cbCoverageView.setSelected(true);
}
else if(cbCoverageView.isSelected() && nbasesInView < MAX_BASES && cbLastSelected != null)
{
cbLastSelected.setSelected(true);
cbLastSelected = null;
}
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
markInsertions.setEnabled(false);
{
scrollBar.setValues(startValue, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.setBlockIncrement(nbasesInView);
}
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}
/**
* Return an Artemis entry from a file
* @param entryFileName
* @param entryGroup
* @return
* @throws NoSequenceException
*/
private Entry getEntry(final String entryFileName, final EntryGroup entryGroup)
throws NoSequenceException
{
final Document entry_document = DocumentFactory.makeDocument(entryFileName);
final EntryInformation artemis_entry_information =
Options.getArtemisEntryInformation();
//System.out.println(entryFileName);
final uk.ac.sanger.artemis.io.Entry new_embl_entry =
EntryFileDialog.getEntryFromFile(null, entry_document,
artemis_entry_information,
false);
if(new_embl_entry == null) // the read failed
return null;
Entry entry = null;
try
{
if(entryGroup.getSequenceEntry() != null)
bases = entryGroup.getSequenceEntry().getBases();
else
entry = new Entry(bases,new_embl_entry);
entryGroup.add(entry);
}
catch(OutOfRangeException e)
{
new MessageDialog(null, "read failed: one of the features in " +
entryFileName + " has an out of range " +
"location: " + e.getMessage());
}
return entry;
}
private boolean isShowScale()
{
return (feature_display == null ? true : false);
}
public JScrollPane getJspView()
{
return jspView;
}
/**
* Handle a mouse drag event on the drawing canvas.
**/
if(event.getButton() == MouseEvent.BUTTON3 || bases == null)
tjc
committed
highlightSAMRecord = null;
if(event.getClickCount() > 1)
{
getSelection().clear();
repaint();
return;
}
highlightRange(event,
MouseEvent.BUTTON1_DOWN_MASK & MouseEvent.BUTTON2_DOWN_MASK);
}
/**
*
* @param event
* @param onmask
*/
private void highlightRange(MouseEvent event, int onmask)
{
int start = (int) ( ( (event.getPoint().getX())/pixPerBase) + getBaseAtStartOfView() );
if(start < 1)
start = 1;
if(start > seqLength)
start = seqLength;
if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
dragStart = start;
else if((event.getModifiersEx() & onmask) != onmask)
dragStart = -1;
if(dragStart < 0)
drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
else
drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
getSelection().setMarkerRange(drag_range);
repaint();
/**
* Get the colour for the given read given to it by the coverage plot.
* @param samRecord
* @return
*/
private Color getColourByCoverageColour(SAMRecord samRecord)
{
LineAttributes lines[] = CoveragePanel.getLineAttributes(bamList.size());
int fileIndex = 0;
if(bamList.size()>1)
fileIndex = (Integer) samRecord.getAttribute("FL");
return lines[fileIndex].getLineColour();
}
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protected int getMaxBases()
{
return MAX_BASES;
}
protected void setMaxBases(int max)
{
MAX_BASES = max;
}
private boolean isStackView()
{
return cbStackView.isSelected();
}
private boolean isPairedStackView()
{
return cbPairedStackView.isSelected();
}
private boolean isStrandStackView()
{
return cbStrandStackView.isSelected();
}
private boolean isCoverageView(float pixPerBase)
{
if(isBaseAlignmentView(pixPerBase))
return false;
return cbCoverageView.isSelected();
}
private boolean isIsizeStackView()
{
return cbIsizeStackView.isSelected();
}
private boolean isBaseAlignmentView(float pixPerBase)
{
if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
return true;
return false;
}
private JCheckBoxMenuItem getSelectedCheckBoxMenuItem()
{
if(isStackView())
return cbStackView;
if(isPairedStackView())
return cbPairedStackView;
if(isStrandStackView())
return cbStrandStackView;
if(isIsizeStackView())
return cbIsizeStackView;
return cbCoverageView;
}
private Selection getSelection()
{
return selection;
}
protected int getBasesInView()
{
return nbasesInView;
}
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private String getVersion()
{
final ClassLoader cl = this.getClass().getClassLoader();
try
{
String line;
InputStream in = cl.getResourceAsStream("etc/versions");
BufferedReader reader = new BufferedReader(new InputStreamReader(in));
while((line = reader.readLine()) != null)
{
if(line.startsWith("BamView"))
return line.substring( "BamView".length() ).trim();
}
reader.close();
in.close();
}
catch (Exception ex)
{
}
return null;
}
public void displayAdjustmentValueChanged(final DisplayAdjustmentEvent event)
if(event.getType() == DisplayAdjustmentEvent.REV_COMP_EVENT &&
event.isRevCompDisplay())
JOptionPane.showMessageDialog(this,
"Flipping the display is not supported by BamView.", "Warning",
JOptionPane.WARNING_MESSAGE);
if(!asynchronous)
{
// if not asynchronous
displayAdjustmentWork(event);
return;
}
public Object construct()
{
try
{
Thread.sleep(500);
}
catch (InterruptedException e)
{
e.printStackTrace();
}
if(event.getStart() != ((FeatureDisplay)event.getSource()).getForwardBaseAtLeftEdge())
{
waitingFrame.showWaiting("waiting...", mainPanel);
waitingFrame.setVisible(false);
return null;
}
};
worker.start();
/**
* Carry out the display agjustment event action.
* @param event
*/
private void displayAdjustmentWork(final DisplayAdjustmentEvent event)
{
if(event.getType() == DisplayAdjustmentEvent.SCALE_ADJUST_EVENT)
{
laststart = -1;