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else if(interval > 16000)
drawTicks(g2, start, end, pixPerBase, 3200);
else if(interval > 4000)
drawTicks(g2, start, end, pixPerBase, 800);
else if(interval > 1000)
drawTicks(g2, start, end, pixPerBase, 400);
drawTicks(g2, start, end, pixPerBase, 100);
}
private void drawTicks(Graphics2D g2, int start, int end, float pixPerBase, int division)
{
int markStart = (Math.round(start/division)*division);
if(markStart < 1)
markStart = 1;
int sm = markStart-(division/2);
if(sm > start)
g2.drawLine((int)(sm*pixPerBase), getHeight()-14,(int)(sm*pixPerBase), getHeight()-12);
for(int m=markStart; m<end; m+=division)
{
g2.drawString(Integer.toString(m), m*pixPerBase, getHeight()-1);
g2.drawLine((int)(m*pixPerBase), getHeight()-14,(int)(m*pixPerBase), getHeight()-11);
sm = m+(division/2);
if(sm < end)
g2.drawLine((int)(sm*pixPerBase), getHeight()-14,(int)(sm*pixPerBase), getHeight()-12);
/**
* Draw a y-scale for inferred size (isize) of reads.
* @param g2
* @param start
* @param pixPerBase
* @param xScaleHeight
*/
private void drawYScale(Graphics2D g2, int start, float pixPerBase, int xScaleHeight)
int maxY = getPreferredSize().height-xScaleHeight;
int xpos = (int) (pixPerBase*start);
for(int i=100; i<maxY; i+=100)
{
int ypos = getHeight()-i-xScaleHeight;
g2.drawLine(xpos, ypos, xpos+2, ypos);
g2.drawString(Integer.toString(i), xpos+3, ypos);
/**
* Draw a given read.
* @param g2
* @param thisRead
* @param pixPerBase
* @param stroke
* @param ypos
*/
int thisEnd = thisRead.getAlignmentEnd()-1;
g2.drawLine((int)(thisStart * pixPerBase), ypos,
(int)(thisEnd * pixPerBase), ypos);
// test if the mouse is over this read
if(lastMousePoint != null)
{
if(lastMousePoint.getY()+2 > ypos && lastMousePoint.getY()-2 < ypos)
if(lastMousePoint.getX() > thisStart * pixPerBase &&
lastMousePoint.getX() < thisEnd * pixPerBase)
{
mouseOverSAMRecord = thisRead;
}
}
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/**
* Highlight a selected range
* @param g2
* @param pixPerBase
* @param start
* @param end
*/
private void drawSelectionRange(Graphics2D g2, float pixPerBase, int start, int end)
{
if(getSelection() != null)
{
Range selectedRange = getSelection().getSelectionRange();
if(selectedRange != null)
{
int rangeStart = selectedRange.getStart();
int rangeEnd = selectedRange.getEnd();
if(end < rangeStart || start > rangeEnd)
return;
int x = (int) (pixPerBase*(rangeStart-1));
int width = (int) (pixPerBase*(rangeEnd-rangeStart+1));
g2.setColor(Color.pink);
g2.fillRect(x, 0, width, getHeight());
}
}
}
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/**
* Draw a translucent line
* @param g2
* @param start
* @param end
* @param ypos
*/
private void drawTranslucentLine(Graphics2D g2, int start, int end, int ypos)
{
Composite origComposite = g2.getComposite();
g2.setComposite(translucent);
g2.drawLine(start, ypos, end, ypos);
g2.setComposite(origComposite);
}
/**
* Draw a translucent line
* @param g2
* @param start
* @param end
* @param ypos
*/
private void drawTranslucentJointedLine(Graphics2D g2, int start, int end, int ypos)
{
Composite origComposite = g2.getComposite();
g2.setComposite(translucent);
int mid = (int) ((end-start)/2.f)+start;
//g2.drawLine(start, ypos, end, ypos);
g2.drawLine(start, ypos, mid, ypos-5);
g2.drawLine(mid, ypos-5, end, ypos);
g2.setComposite(origComposite);
}
/**
* Display the SNPs for the given read.
* @param g2
* @param thisRead
* @param pixPerBase
* @param ypos
*/
private void showSNPsOnReads(Graphics2D g2, SAMRecord thisRead,
float pixPerBase, int ypos)
{
int thisStart = thisRead.getAlignmentStart();
int thisEnd = thisRead.getAlignmentEnd();
// use alignment blocks of the contiguous alignment of
// subsets of read bases to a reference sequence
List<AlignmentBlock> blocks = thisRead.getAlignmentBlocks();
char[] refSeq = bases.getSubSequenceC(
new Range(thisStart, thisEnd), Bases.FORWARD);
byte[] readSeq = thisRead.getReadBases();
for(int i=0; i<blocks.size(); i++)
AlignmentBlock block = blocks.get(i);
for(int j=0; j<block.getLength(); j++)
int readPos = block.getReadStart()-1+j;
int refPos = block.getReferenceStart()+j;
if (Character.toUpperCase(refSeq[refPos-thisStart]) != readSeq[readPos])
{
g2.drawLine((int) ((refPos) * pixPerBase), ypos + 2,
(int) ((refPos) * pixPerBase), ypos - 2);
g2.setColor(col);
}
catch (OutOfRangeException e)
{
e.printStackTrace();
/**
* Add the alignment view to the supplied <code>JPanel</code> in
* a <code>JScrollPane</code>.
* @param mainPanel panel to add the alignment to
* @param autohide automatically hide the top panel containing the buttons
*/
public void addJamToPanel(final JPanel mainPanel,
final boolean autohide,
final FeatureDisplay feature_display)
if(feature_display != null)
{
this.feature_display = feature_display;
this.selection = feature_display.getSelection();
topPanel = new JPanel(new FlowLayout(FlowLayout.LEADING, 0, 0));
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
final JCheckBox buttonAutoHide = new JCheckBox("Hide", autohide);
buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
handleCanvasMouseDragOrClick(event);
}
public void mouseMoved(MouseEvent e)
{
int thisHgt = HEIGHT;
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
if (y < thisHgt)
}
mainPanel.repaint();
mainPanel.revalidate();
}
};
addMouseMotionListener(mouseMotionListener);
combo = new JComboBox(seqNames);
combo.setEditable(false);
combo.addItemListener(new ItemListener()
{
public void itemStateChanged(ItemEvent e)
{
laststart = -1;
lastend = -1;
setZoomLevel(JamView.this.nbasesInView);
}
});
goTo.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
try
{
int basePosition = Integer.parseInt(baseText.getText());
goToBasePosition(basePosition);
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(JamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
JButton zoomIn = new JButton("-");
Insets ins = new Insets(1,1,1,1);
zoomIn.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
int startBase = getBaseAtStartOfView();
setZoomLevel((int) (JamView.this.nbasesInView * 1.1));
goToBasePosition(startBase);
}
});
JButton zoomOut = new JButton("+");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
int startBase = getBaseAtStartOfView();
setZoomLevel((int) (JamView.this.nbasesInView * .9));
goToBasePosition(startBase);
}
});
jspView = new JScrollPane(this,
JScrollPane.VERTICAL_SCROLLBAR_AS_NEEDED,
setDisplay(1, nbasesInView, null);
mainPanel.setLayout(new BorderLayout());
if(topPanel instanceof JPanel)
mainPanel.add(topPanel, BorderLayout.NORTH);
mainPanel.add(jspView, BorderLayout.CENTER);
coveragePanel = new CoveragePanel();
mainPanel.add(coveragePanel, BorderLayout.SOUTH);
coveragePanel.setPreferredSize(new Dimension(900, 100));
coveragePanel.setVisible(false);
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
public void keyPressed(final KeyEvent event)
setZoomLevel((int) (JamView.this.nbasesInView * 1.1));
setZoomLevel((int) (JamView.this.nbasesInView * .9));
addMouseListener(new PopupListener());
public void focusGained(FocusEvent fe){}
public void focusLost(FocusEvent fe){}
JCheckBoxMenuItem checkBoxSingle = new JCheckBoxMenuItem("Single Reads");
checkBoxSingle.addActionListener(new ActionListener()
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public void actionPerformed(ActionEvent e)
{
repaint();
isSingle = !isSingle;
}
});
view.add(checkBoxSingle);
JCheckBoxMenuItem checkBoxSNPs = new JCheckBoxMenuItem("SNPs");
checkBoxSNPs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (isSNPs && bases == null)
{
JOptionPane.showMessageDialog(null,
"No reference sequence supplied to identify SNPs.", "SNPs",
JOptionPane.INFORMATION_MESSAGE);
}
isSNPs = !isSNPs;
repaint();
}
});
view.add(checkBoxSNPs);
final JCheckBoxMenuItem checkBoxStackView = new JCheckBoxMenuItem("Stack View");
final JCheckBoxMenuItem checkBoxPairedStackView = new JCheckBoxMenuItem("Paired Stack View");
checkBoxStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
lastend = -1;
isStackView = !isStackView;
if(isStackView)
{
isPairedStackView = !isStackView;
checkBoxPairedStackView.setSelected(!isStackView);
}
checkBoxPairedStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
lastend = -1;
isPairedStackView = !isPairedStackView;
if(isPairedStackView)
{
isStackView = !isPairedStackView;
checkBoxStackView.setSelected(!isPairedStackView);
}
repaint();
}
});
view.add(checkBoxPairedStackView);
JCheckBoxMenuItem checkBoxCoverage = new JCheckBoxMenuItem("Coverage");
checkBoxCoverage.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isCoverage = !isCoverage;
coveragePanel.setVisible(isCoverage);
repaint();
}
});
view.add(checkBoxCoverage);
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view.add(new JSeparator());
JMenu maxHeightMenu = new JMenu("Plot Height");
view.add(maxHeightMenu);
final String hgts[] =
{"500", "800", "1000", "1500", "2500", "5000"};
ButtonGroup bgroup = new ButtonGroup();
for(int i=0; i<hgts.length; i++)
{
final String hgt = hgts[i];
final JCheckBoxMenuItem maxHeightMenuItem = new JCheckBoxMenuItem(hgt);
bgroup.add(maxHeightMenuItem);
maxHeightMenuItem.setSelected(hgts[i].equals(Integer.toString(maxHeight)));
maxHeightMenu.add(maxHeightMenuItem);
maxHeightMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(maxHeightMenuItem.isSelected())
maxHeight = Integer.parseInt(hgt);
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
}
});
}
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
private int getBaseAtStartOfView()
{
String refName = (String) combo.getSelectedItem();
int seqLength = seqLengths.get(refName);
double x = jspView.getViewport().getViewRect().getX();
return (int) (getMaxBasesInPanel(seqLength) * ( x / getWidth())) + 1;
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
private void setZoomLevel(final int nbasesInView)
{
this.nbasesInView = nbasesInView;
//System.out.println("setZoomLevel "+nbasesInView+" "+getBaseAtStartOfView());
String refName = (String) combo.getSelectedItem();
int seqLength = seqLengths.get(refName);
float pixPerBase = getPixPerBaseByBasesInView();
if(pixPerBase*3 > ALIGNMENT_PIX_PER_BASE)
{
pixPerBase = ALIGNMENT_PIX_PER_BASE;
jspView.getVerticalScrollBar().setValue(0);
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
}
else if(jspView != null)
{
jspView.setColumnHeaderView(null);
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
showBaseAlignment = false;
}
Dimension d = new Dimension();
d.setSize((getMaxBasesInPanel(seqLength)*pixPerBase), maxHeight);
}
/**
* Set the ViewPort so it starts at the given base position.
* @param base
*/
String refName = (String) combo.getSelectedItem();
int seqLength = seqLengths.get(refName);
p.x = Math.round(width*((float)(base-1)/(getMaxBasesInPanel(seqLength))));
//System.out.println("goToBasePosition "+base);
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
String refName = (String) combo.getSelectedItem();
int seqLength = seqLengths.get(refName);
pixPerBase = this.getPixPerBaseByWidth(); // getPixPerBaseByBasesInView();
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(pixPerBase*3 > ALIGNMENT_PIX_PER_BASE)
d.setSize((getMaxBasesInPanel(seqLength)*pixPerBase), maxHeight);
//System.out.println("setDisplay() "+d.getWidth());
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}
/**
* Return an Artemis entry from a file
* @param entryFileName
* @param entryGroup
* @return
* @throws NoSequenceException
*/
private Entry getEntry(final String entryFileName, final EntryGroup entryGroup)
throws NoSequenceException
{
final Document entry_document = DocumentFactory.makeDocument(entryFileName);
final EntryInformation artemis_entry_information =
Options.getArtemisEntryInformation();
//System.out.println(entryFileName);
final uk.ac.sanger.artemis.io.Entry new_embl_entry =
EntryFileDialog.getEntryFromFile(null, entry_document,
artemis_entry_information,
false);
if(new_embl_entry == null) // the read failed
return null;
Entry entry = null;
try
{
if(entryGroup.getSequenceEntry() != null)
bases = entryGroup.getSequenceEntry().getBases();
else
entry = new Entry(bases,new_embl_entry);
entryGroup.add(entry);
}
catch(OutOfRangeException e)
{
new MessageDialog(null, "read failed: one of the features in " +
entryFileName + " has an out of range " +
"location: " + e.getMessage());
}
return entry;
}
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private boolean isShowScale()
{
return showScale;
}
public void setShowScale(boolean showScale)
{
this.showScale = showScale;
}
public JScrollPane getJspView()
{
return jspView;
}
public void setBases(Bases bases)
{
this.bases = bases;
}
/**
* Remove JScrollPane border
*/
public void removeBorder()
{
Border empty = new EmptyBorder(0,0,0,0);
jspView.setBorder(empty);
}
/**
* Handle a mouse drag event on the drawing canvas.
**/
private void handleCanvasMouseDragOrClick(final MouseEvent event)
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if(event.isShiftDown() || event.getButton() == MouseEvent.BUTTON3)
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if(event.getClickCount() > 1)
{
getSelection().clear();
repaint();
return;
}
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int onmask = MouseEvent.BUTTON1_DOWN_MASK;
String refName = (String) combo.getSelectedItem();
int seqLength = seqLengths.get(refName);
float pixPerBase = getPixPerBaseByWidth();
int start = (int) ( (event.getPoint().getX()/pixPerBase) + 1.f );
if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
dragStart = start;
else if((event.getModifiersEx() & onmask) != onmask)
dragStart = -1;
if(start < 1)
start = 1;
if(start > seqLength)
start = seqLength;
if(dragStart < 0)
drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
else
drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
getSelection().setMarkerRange(drag_range);
repaint();
}
}
private Selection getSelection()
{
return selection;
}
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private class CoveragePanel extends JPanel
{
private static final long serialVersionUID = 1L;
private int start;
private int end;
private float pixPerBase;
public CoveragePanel()
{
super();
setBackground(Color.white);
}
/**
* Override
*/
protected void paintComponent(Graphics g)
{
super.paintComponent(g);
Graphics2D g2 = (Graphics2D)g;
if(readsInView == null)
return;
int windowSize = 10;
int nBins = Math.round((end-start+1.f)/windowSize);
int coverage[] = new int[nBins];
coverage[i] = 0;
for(int i=0; i<readsInView.size(); i++)
{
SAMRecord thisRead = readsInView.get(i);
int length = thisRead.getReadLength();
for(int j=0; j<length;j++)
{
int bin =
Math.round(((thisRead.getAlignmentStart()-start) + j) / windowSize);
if(bin < 0 || bin > coverage.length-1)
continue;
coverage[bin]+=1;
if(coverage[bin] > max)
max = coverage[bin];
}
}
g2.setColor(lightBlue);
GeneralPath shape = new GeneralPath();
shape.moveTo(0,getHeight());
for(int i=0; i<coverage.length; i++)
{
float xpos = ((i*(windowSize)) - windowSize/2.f)*pixPerBase;
shape.lineTo(xpos,
getHeight() - (((float)coverage[i]/(float)max)*getHeight()));
}
shape.lineTo(getWidth(),getHeight());
g2.fill(shape);
String maxStr = Integer.toString(max);
FontMetrics fm = getFontMetrics(getFont());
g2.setColor(Color.black);
tjc
committed
int xpos = getWidth() - fm.stringWidth(maxStr) -
jspView.getVerticalScrollBar().getWidth();
g2.drawString(maxStr, xpos, fm.getHeight());
}
protected void setStartAndEnd(int start, int end)
{
this.start = start;
this.end = end;
}
public void setPixPerBase(float pixPerBase)
{
this.pixPerBase = pixPerBase;
}
}
public Ruler()
{
super();
setPreferredSize(new Dimension(getPreferredSize().width, 15));
setBackground(Color.white);
setFont(getFont().deriveFont(11.f));
}
public void paintComponent(Graphics g)
{
super.paintComponent(g);
Graphics2D g2 = (Graphics2D)g;
drawBaseScale(g2, start, end, 12);
}
private void drawBaseScale(Graphics2D g2, int start, int end, int ypos)
{
int startMark = (((int)(start/10))*10)+1;
for(int i=startMark; i<end; i+=10)
{
g2.drawString(Integer.toString(i), xpos, ypos);
xpos+=(ALIGNMENT_PIX_PER_BASE/2);
g2.drawLine(xpos, ypos+1, xpos, ypos+5);
}
}
/**
* Popup menu listener
*/
class PopupListener extends MouseAdapter
{
public void mouseClicked(MouseEvent e)
{
JamView.this.requestFocus();
}
public void mousePressed(MouseEvent e)
{
maybeShowPopup(e);
}
public void mouseReleased(MouseEvent e)
{
maybeShowPopup(e);
}
private void maybeShowPopup(MouseEvent e)
{
if(e.isPopupTrigger())
{
if(popup == null)
{
popup = new JPopupMenu();
createViewMenu(popup);
}
popup.show(e.getComponent(),
e.getX(), e.getY());
SAMRecord pr1 = (SAMRecord) o1;
SAMRecord pr2 = (SAMRecord) o2;
int cmp = pr1.getReadName().compareTo(pr2.getReadName());
if(cmp == 0)
{
if(pr1.getAlignmentStart() < pr2.getAlignmentStart())
return -1;
else
return 1;
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class PairedReadComparator implements Comparator<Object>
{
public int compare(Object o1, Object o2)
{
PairedRead pr1 = (PairedRead) o1;
PairedRead pr2 = (PairedRead) o2;
int start1 = pr1.sam1.getAlignmentStart();
if(pr1.sam1.getAlignmentEnd() < start1)
start1 = pr1.sam1.getAlignmentEnd();
int start2 = pr2.sam1.getAlignmentStart();
if(pr2.sam1.getAlignmentEnd() < start2)
start2 = pr1.sam1.getAlignmentEnd();
return start1-start2;
}
}
class PairedRead
{
SAMRecord sam1;
SAMRecord sam2;
}
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public Dimension getPreferredScrollableViewportSize()
{
return getPreferredSize();
}
public int getScrollableBlockIncrement(Rectangle visibleRect,
int orientation, int direction)
{
if (orientation == SwingConstants.HORIZONTAL)
return visibleRect.width - maxUnitIncrement;
else
return visibleRect.height - maxUnitIncrement;
}
public boolean getScrollableTracksViewportHeight()
{
return false;
}
public boolean getScrollableTracksViewportWidth()
{
return false;
}
public int getScrollableUnitIncrement(Rectangle visibleRect, int orientation,
int direction)
{
//Get the current position.
int currentPosition = 0;
if (orientation == SwingConstants.HORIZONTAL)
currentPosition = visibleRect.x;
else
currentPosition = visibleRect.y;
//Return the number of pixels between currentPosition
//and the nearest tick mark in the indicated direction.
if (direction < 0)
{
int newPosition = currentPosition -
(currentPosition / maxUnitIncrement)
* maxUnitIncrement;
return (newPosition == 0) ? maxUnitIncrement : newPosition;
}
else