AlignmentViewer.java

/* AlignmentViewer.java
 *
 * created: Mon Jul 12 1999
 *
 * This file is part of Artemis
 *
 * Copyright (C) 1999,2000,2001  Genome Research Limited
 *
 * This program is free software; you can redistribute it and/or
 * modify it under the terms of the GNU General Public License
 * as published by the Free Software Foundation; either version 2
 * of the License, or (at your option) any later version.
 *
 * This program is distributed in the hope that it will be useful,
 * but WITHOUT ANY WARRANTY; without even the implied warranty of
 * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
 * GNU General Public License for more details.
 *
 * You should have received a copy of the GNU General Public License
 * along with this program; if not, write to the Free Software
 * Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
 *
 * $Header: //tmp/pathsoft/artemis/uk/ac/sanger/artemis/components/AlignmentViewer.java,v 1.43 2008-11-28 17:51:09 tjc Exp $
 */

package uk.ac.sanger.artemis.components;

import uk.ac.sanger.artemis.*;
import uk.ac.sanger.artemis.sequence.Strand;
import uk.ac.sanger.artemis.sequence.Bases;
import uk.ac.sanger.artemis.sequence.SequenceChangeListener;
import uk.ac.sanger.artemis.sequence.SequenceChangeEvent;
import uk.ac.sanger.artemis.util.StringVector;
import uk.ac.sanger.artemis.io.Range;
import uk.ac.sanger.artemis.io.RangeVector;

import java.awt.*;
import java.awt.event.*;
import java.util.Vector;
import java.util.Comparator;
import java.util.Arrays;
import javax.swing.*;
import java.io.FileWriter;
import java.io.IOException;

/**
 *  This component shows an alignment of two sequences using the data from a
 *  ComparisonData object.
 *
 *  @author Kim Rutherford
 *  @version $Id: AlignmentViewer.java,v 1.43 2008-11-28 17:51:09 tjc Exp $
 **/

public class AlignmentViewer extends CanvasPanel
    implements SequenceChangeListener 
{
  private static final long serialVersionUID = 1L;

  private Image offscreen;

  /** Comparison data that will be displayed in this component. */
  final private ComparisonData comparison_data;

  /** 
   *  All the AlignMatch objects from comparison_data (possibly in a
   *  different order.
   **/
  private AlignMatch[] all_matches = null;

  /**
   *  This is the last DisplayAdjustmentEvent reference that was passed to
   *  setSubjectSeqeuencePosition().
   **/
  private DisplayAdjustmentEvent last_subject_event;

  /**
   *  This is the last DisplayAdjustmentEvent reference that was passed to
   *  setQuerySeqeuencePosition().
   **/
  private DisplayAdjustmentEvent last_query_event;

  /** FeatureDisplay that is above this component.  (From the constructor). */
  private FeatureDisplay subject_feature_display;

  /** FeatureDisplay that is below this component.  (From the constructor). */
  private FeatureDisplay query_feature_display;

  /**
   *  Set by the constructor to be the original forward strand for the subject
   *  sequence.  This is use to determine whether to subject sequence has been
   *  reverse-complemented or not.
   **/
  private Strand orig_subject_forward_strand;

  /**
   *  Set by the constructor to be the original forward strand for the query
   *  sequence.  This is use to determine whether to query sequence has been
   *  reverse-complemented or not.
   **/
  private Strand orig_query_forward_strand;

  /**
   *  Set by the constructor to be the original reverse strand for the subject
   *  sequence.
   **/
  private Strand orig_subject_reverse_strand;

  /**
   *  Set by the constructor to be the original reverse strand for the query
   *  sequence.
   **/
  private Strand orig_query_reverse_strand;

  /** One of the two Entry objects that we are comparing. */
  final private EntryGroup subject_entry_group;

  /** One of the two Entry objects that we are comparing. */
  final private EntryGroup query_entry_group;

  /** Selected matches.  null means no matches are selected. */
  private AlignMatchVector selected_matches = null;

  /**
   *  The objects that are listening for AlignmentSelectionChangeEvents.
   **/
  private Vector selection_change_listeners = new Vector();

  /**
   *  The number of shades of red and blue to use for percentage ID colouring.
   **/
  private static int NUMBER_OF_SHADES = 13;

  /** Reds used to display the percent identity of matches.  */
  private Color[] red_percent_id_colours;

  /** Blues used to display the percent identity of matches. */
  private Color[] blue_percent_id_colours;

  /** Scroll bar used to set the minimum length of the visible matches. */
  private JScrollBar scroll_bar = null;

  /** Matches with scores below this value will not be shown. */
  private int minimum_score = 0;

  /** Matches with scores above this value will not be shown. */
  private int maximum_score = 99999999;

  /**
   *  Matches with percent id values below this number will not be shown.
   **/
  private int minimum_percent_id = 0;

  /**
   *  Matches with percent id values above this number will not be shown.
   **/
  private int maximum_percent_id = 100;

  /**
   *  True if we should offer to flip the query sequence when the user
   *  double clicks on a flipped match.
   **/
  private boolean offer_to_flip_flag = false;

  /**
   *  If true ignore self matches (ie query start == subject start && query
   *  end == subject end)
   **/
  private boolean ignore_self_match_flag = false;

  /** Vector of those objects that are listening for AlignmentEvents */
  private java.util.Vector alignment_event_listeners =
    new java.util.Vector ();

  /**
   *  If true then the FeatureDisplays above and below this AlignmentViewer
   *  should scroll together.
   **/
  private boolean displays_are_locked = true;

  /**
   *  Setting this to true will temporarily disable selectFromQueryRange() and
   *  selectFromSubjectRange() until enableSelection().  This is need to allow
   *  the selections of the top and bottom FeatureDisplays to be set without
   *  changing which AlignMatches are selected.
   **/
  private boolean disable_selection_from_ranges = false;

  /** user defined colours */
  private boolean reverseMatchColour = false;
  /** colour for reverse matches */
  private Color revMatchColour = Color.blue;
  /** colour for matches         */
  private Color matchColour    = Color.red;

  /**
   *  Create a new AlignmentViewer for the given entries.
   *  @param subject_feature_display The FeatureDisplay that is above this
   *    component.
   *  @param query_feature_display The FeatureDisplay that is below this
   *    component.
   *  @param comparison_data Provides the AlignMatch objects that will be
   *    displayed.
   **/
  public AlignmentViewer(final FeatureDisplay subject_feature_display,
                         final FeatureDisplay query_feature_display,
                         final ComparisonData comparison_data) 
  {
    this.subject_feature_display = subject_feature_display;
    this.query_feature_display   = query_feature_display;
    this.comparison_data         = comparison_data;
    this.all_matches             = getComparisonData().getMatches();

    subject_entry_group          = getSubjectDisplay().getEntryGroup();
    query_entry_group            = getQueryDisplay().getEntryGroup();

    final Bases subject_bases = getSubjectForwardStrand().getBases();
    final Bases query_bases   = getQueryForwardStrand().getBases();

    final Selection subject_selection = getSubjectDisplay().getSelection();
    final Selection query_selection = getQueryDisplay().getSelection();

    final SelectionChangeListener subject_listener =
      new SelectionChangeListener() 
    {
      JFrame frame = null;
      public void selectionChanged(SelectionChangeEvent event) 
      {
        if(frame == null)
          frame = subject_feature_display.getParentFrame();
        if(!frame.isVisible())
          return;

        final RangeVector ranges = subject_selection.getSelectionRanges();
        selectFromSubjectRanges(ranges);
      }
    };

    final SelectionChangeListener query_listener =
      new SelectionChangeListener() 
    {
      JFrame frame = null;
      public void selectionChanged (SelectionChangeEvent event) 
      {
        if(frame == null)
          frame = query_feature_display.getParentFrame();
        if(!frame.isVisible())
          return;

        final RangeVector ranges = query_selection.getSelectionRanges ();
        selectFromQueryRanges(ranges);
      }
    };

    makeColours();

    subject_selection.addSelectionChangeListener(subject_listener);
    query_selection.addSelectionChangeListener(query_listener);

    subject_bases.addSequenceChangeListener(this, 0);
    query_bases.addSequenceChangeListener(this, 0);

    orig_subject_forward_strand = getSubjectForwardStrand();
    orig_query_forward_strand   = getQueryForwardStrand();

    addMouseListener(new MouseAdapter() 
    {
      public void mousePressed(final MouseEvent event) 
      {
        // on windows we have to check isPopupTrigger in mouseReleased(),
        // but do it in mousePressed() on UNIX
        if(isMenuTrigger(event)) 
          popupMenu(event);
        else 
          handleCanvasMousePress(event);
      }
    });

    addMouseMotionListener(new MouseMotionAdapter() 
    {
      public void mouseDragged(final MouseEvent event) 
      {
        if(isMenuTrigger(event))
          return;

        if(!modifiersForLockToggle(event))
        {
          if(!event.isShiftDown()) 
          {
            selected_matches = null;
            toggleSelection (event.getPoint());
          }
          repaint();
        }
      }
    });

    scroll_bar = new JScrollBar(Scrollbar.VERTICAL);
    scroll_bar.setValues(1, 1, 1, 1000);
    scroll_bar.setBlockIncrement(10);

    scroll_bar.addAdjustmentListener(new AdjustmentListener() 
    {
      public void adjustmentValueChanged(AdjustmentEvent e) 
      {
        repaint();
      }
    });

    maximum_score = getComparisonData().getMaximumScore();

    add(scroll_bar, "East");
    setBackground(Color.white);
  }

  /**
   *  Returns true if and only if the given MouseEvent should toggle the lock
   *  displays toggle.
   **/
  private boolean modifiersForLockToggle(final MouseEvent event) 
  {
    return(event.getModifiers() & InputEvent.BUTTON2_MASK) != 0 ||
      event.isAltDown();
  }

  /**
   *  Select those matches that overlap the given range on the subject
   *  sequence.
   **/
  public void selectFromSubjectRanges(final RangeVector select_ranges) 
  {
    if(disable_selection_from_ranges) 
      return;

    selected_matches = null;
    final int all_matches_length = all_matches.length;
    final int select_ranges_size = select_ranges.size();

    final Strand current_subject_fwd_strand =
                              getSubjectForwardStrand();

    final int subject_length = current_subject_fwd_strand.getSequenceLength();

    for(int match_index = 0; match_index < all_matches_length; ++match_index)
    {
      final AlignMatch this_match = all_matches[match_index];

      if(!isVisible(this_match))
        continue;

      int subject_sequence_start = getRealSubjectSequenceStart(this_match,
          subject_length,
          (getOrigSubjectForwardStrand() != current_subject_fwd_strand));
      int subject_sequence_end = getRealSubjectSequenceEnd(this_match,
          subject_length,
          (getOrigSubjectForwardStrand() != current_subject_fwd_strand));

      if(subject_sequence_end < subject_sequence_start)
      {
        final int tmp = subject_sequence_start;
        subject_sequence_start = subject_sequence_end;
        subject_sequence_end = tmp;
      }

      for(int range_index = 0; range_index < select_ranges_size; ++range_index)
      {
        final Range select_range = (Range) select_ranges.elementAt(range_index);
        final int select_range_start = select_range.getStart();
        final int select_range_end = select_range.getEnd();

        if(select_range_start < subject_sequence_start
            && select_range_end < subject_sequence_start)
          continue;

        if(select_range_start > subject_sequence_end &&
           select_range_end   > subject_sequence_end) 
          continue;

        if(selected_matches == null) 
          selected_matches = new AlignMatchVector();

        //if(!selected_matches.contains(this_match)) 
        selected_matches.add(this_match);
        break;
      }
    }

    if(selected_matches != null)
      selectionChanged();
    else
      repaint();
  }

  /**
   *  Select those matches that overlap the given range on the query sequence.
   **/
  public void selectFromQueryRanges(final RangeVector select_ranges) 
  {
    if(disable_selection_from_ranges) 
      return;

    selected_matches = null;
    final int select_ranges_size = select_ranges.size();
    final int all_matches_length = all_matches.length;
    final Strand current_query_forward_strand = getQueryForwardStrand();
    final int query_length =
          current_query_forward_strand.getSequenceLength();

    for(int match_index = 0; match_index < all_matches_length; ++match_index)
    {
      final AlignMatch this_match = all_matches[match_index];

      if(!isVisible(this_match))
        continue;

      int query_sequence_start = getRealQuerySequenceStart(this_match,
          query_length,
          (getOrigQueryForwardStrand() != current_query_forward_strand));
      int query_sequence_end = getRealQuerySequenceEnd(this_match,
          query_length,
          (getOrigQueryForwardStrand() != current_query_forward_strand));

      if(query_sequence_end < query_sequence_start)
      {
        final int tmp = query_sequence_start;
        query_sequence_start = query_sequence_end;
        query_sequence_end = tmp;
      }

      for(int range_index = 0; range_index < select_ranges_size; ++range_index)
      {
        final Range select_range = (Range) select_ranges.elementAt(range_index);
        final int select_range_start = select_range.getStart();
        final int select_range_end = select_range.getEnd();
        if(select_range_start < query_sequence_start
            && select_range_end < query_sequence_start) 
          continue;

        if(select_range_start > query_sequence_end &&
           select_range_end > query_sequence_end) 
          continue;

        if(selected_matches == null) 
          selected_matches = new AlignMatchVector();

        //if(!selected_matches.contains(this_match)) 
        selected_matches.add(this_match);
        break;
      }
    }
    
    if(selected_matches != null)
      selectionChanged();
    else
      repaint();
  }

  /**
   *  Select the given match and move it to the top of the display.
   **/
  public void setSelection(final AlignMatch match) 
  {
    selected_matches = new AlignMatchVector();
    selected_matches.add(match);
    selectionChanged();
  }

  /**
   *  This method tells this AlignmentViewer component where the subject
   *  sequence is now.
   **/
  public void setSubjectSequencePosition(final DisplayAdjustmentEvent event) 
  {
    last_subject_event = event;
    repaint();
  }

  /**
   *  This method tells this AlignmentViewer component where the query
   *  sequence is now.
   **/
  public void setQuerySequencePosition(final DisplayAdjustmentEvent event) 
  {
    last_query_event = event;
    repaint();
  }

  /**
   *  Implementation of the SequenceChangeListener interface.  The display is
   *  redrawn if there is an event.
   **/
  public void sequenceChanged(final SequenceChangeEvent event) 
  {
    repaint();
  }

  /**
   *  Return true if and only if the given MouseEvent (a mouse press) should
   *  pop up a JPopupMenu.
   **/
  private boolean isMenuTrigger(final MouseEvent event) 
  {
    if( event.isPopupTrigger() ||
       (event.getModifiers() & InputEvent.BUTTON3_MASK) != 0) 
      return true;
    else 
      return false;
  }

  /**
   *  Popup a menu.
   **/
  private void popupMenu(final MouseEvent event) 
  {
    final JPopupMenu popup = new JPopupMenu();


    final JMenuItem save_matches = new JMenuItem("Save Comparison File...");
    save_matches.addActionListener(new ActionListener()
    {
      public void actionPerformed (ActionEvent _)
      {
        StickyFileChooser fc = new StickyFileChooser();

        int returnVal = fc.showSaveDialog(null);  
        if(returnVal != JFileChooser.APPROVE_OPTION) 
          return;
        else if(fc.getSelectedFile().exists())
        {
          Object[] possibleValues = { "YES", "NO" };
          int select = JOptionPane.showOptionDialog(null, 
                                 fc.getSelectedFile().getName()+"\n"+
                                 "exists. Overwrite?",
                                 "File Exists",
                                 JOptionPane.DEFAULT_OPTION,
                                 JOptionPane.QUESTION_MESSAGE,null,
                                 possibleValues, possibleValues[0]);
          if(select == 1)
            return;  
        }

        try
        {
/*
          if(!fc.getSelectedFile().canWrite())
          {
            JOptionPane.showMessageDialog(null,
                        "Cannot write to "+
                        fc.getSelectedFile().getCanonicalPath(),
                        "Warning",
                        JOptionPane.WARNING_MESSAGE);
            return;
          }
*/
          final FileWriter out_writer = new FileWriter(fc.getSelectedFile());
          final String query = getQueryEntryGroup().getDefaultEntry().getName();
          final String subject = getSubjectEntryGroup().getDefaultEntry().getName();

          for(int i = 0; i < all_matches.length; ++i)
            MSPcrunchComparisonData.writeMatchFromAlignMatch(all_matches[i],
                                               query, subject,
                                               out_writer);
          out_writer.close();
        }
        catch(IOException ioe)
        {
          JOptionPane.showMessageDialog(null,
                        "Error writing out comparison file.", 
                        "Warning",
                        JOptionPane.WARNING_MESSAGE);
          ioe.printStackTrace();
        }
      }
    });
    popup.add(save_matches);
    popup.add(new JSeparator());

    final JMenuItem alignmatch_list_item =
      new JMenuItem("View Selected Matches");

    popup.add(alignmatch_list_item);

    alignmatch_list_item.addActionListener(new ActionListener() 
    {
      public void actionPerformed (ActionEvent _) 
      {
        if(selected_matches == null) 
          new MessageFrame("No matches selected").setVisible (true);
        else 
        {
          final AlignMatchVector matches =
            (AlignMatchVector)selected_matches.clone();

          final AlignMatchViewer viewer =
            new AlignMatchViewer(AlignmentViewer.this, matches);

          viewer.setVisible(true);
        }
      }
    });

    final JMenuItem flip_subject_item =
      new JMenuItem("Flip Subject Sequence");

    popup.add (flip_subject_item);

    flip_subject_item.addActionListener(new ActionListener() 
    {
      public void actionPerformed(ActionEvent _) 
      {
        if(getSubjectDisplay().isRevCompDisplay()) 
          getSubjectDisplay().setRevCompDisplay(false);
        else 
          getSubjectDisplay().setRevCompDisplay(true);
      }
    });

    final JMenuItem flip_query_item =
      new JMenuItem ("Flip Query Sequence");

    popup.add (flip_query_item);

    flip_query_item.addActionListener(new ActionListener() 
    {
      public void actionPerformed(ActionEvent _) 
      {
        if(getQueryDisplay().isRevCompDisplay())
          getQueryDisplay().setRevCompDisplay(false);
        else
          getQueryDisplay().setRevCompDisplay(true);
      }
    });

    final JMenuItem cutoffs_item = new JMenuItem("Set Score Cutoffs ...");
    popup.add(cutoffs_item);

    cutoffs_item.addActionListener(new ActionListener() 
    {
      public void actionPerformed(ActionEvent _) 
      {
        final ScoreChangeListener minimum_listener =
          new ScoreChangeListener() 
        {
          public void scoreChanged(final ScoreChangeEvent event)
          {
            minimum_score = event.getValue();
            repaint();
          }
        };

        final ScoreChangeListener maximum_listener =
          new ScoreChangeListener()
        {
          public void scoreChanged(final ScoreChangeEvent event) 
          {
            maximum_score = event.getValue();
            repaint();
          }
        };

        final ScoreChanger score_changer =
          new ScoreChanger("Score Cutoffs",
                           minimum_listener, maximum_listener,
                           getComparisonData().getMinimumScore(),
                           getComparisonData().getMaximumScore());

        score_changer.setVisible (true);
      }
    });

    final JMenuItem percent_id_cutoffs_item =
      new JMenuItem("Set Percent ID Cutoffs ...");
    popup.add(percent_id_cutoffs_item);

    percent_id_cutoffs_item.addActionListener(new ActionListener () 
    {
      public void actionPerformed(ActionEvent _) 
      {
        final ScoreChangeListener minimum_listener =
          new ScoreChangeListener()
        {
          public void scoreChanged(final ScoreChangeEvent event) 
          {
            minimum_percent_id = event.getValue();
            repaint();
          }
        };

        final ScoreChangeListener maximum_listener =
          new ScoreChangeListener() 
        {
          public void scoreChanged(final ScoreChangeEvent event) 
          {
            maximum_percent_id = event.getValue();
            repaint();
          }
        };

        final ScoreChanger score_changer =
          new ScoreChanger("Percent Identity Cutoffs",
                           minimum_listener, maximum_listener,
                           0, 100);

        score_changer.setVisible(true);
      }
    });


    final JCheckBoxMenuItem lock_item = new JCheckBoxMenuItem("Lock Sequences");
    lock_item.setSelected(displaysAreLocked());
    popup.add(lock_item);

    lock_item.addItemListener(new ItemListener()
    {
      public void itemStateChanged(ItemEvent e)
      {
        if(lock_item.isSelected())
          lockDisplays();
        else
          unlockDisplays();
      }
    });

    popup.addSeparator();

    final JCheckBoxMenuItem sameColour = 
       new JCheckBoxMenuItem("Colour reverse & forward matches the same",reverseMatchColour);
    sameColour.addItemListener(new ItemListener()
    {
      public void itemStateChanged(ItemEvent e)
      {
        reverseMatchColour = sameColour.getState();
        repaint();
      }
    });
    popup.add(sameColour);

    JMenuItem colourMatches = new JMenuItem("Colour matches...");
    colourMatches.addActionListener(new ActionListener()
    {
      public void actionPerformed(ActionEvent event)
      {
        ColorChooserShades shades = createColours("Colour Matches", 
                                                  red_percent_id_colours[NUMBER_OF_SHADES-1]);
        if(shades != null)
        {
          red_percent_id_colours = shades.getDefinedColour();
          repaint();
        }
      }
    });
    popup.add(colourMatches);

    if(!reverseMatchColour)
    {
      JMenuItem colourRevMatches = new JMenuItem("Colour reverse matches...");
      colourRevMatches.addActionListener(new ActionListener()
      {
        public void actionPerformed(ActionEvent event)
        {
          ColorChooserShades shades = createColours("Colour Reverse Matches",
                                                   blue_percent_id_colours[NUMBER_OF_SHADES-1]);
          if(shades != null)
          {
            blue_percent_id_colours = shades.getDefinedColour();
            repaint();
          }
        }
      });
      popup.add(colourRevMatches);
    }

    popup.addSeparator();

    final JCheckBoxMenuItem offer_to_flip_item =
      new JCheckBoxMenuItem("Offer To RevComp", offer_to_flip_flag);

    offer_to_flip_item.addItemListener(new ItemListener() 
    {
      public void itemStateChanged(ItemEvent e) 
      {
        offer_to_flip_flag = !offer_to_flip_flag;
      }
    });

    popup.add(offer_to_flip_item);

    final JCheckBoxMenuItem ignore_self_match_item =
      new JCheckBoxMenuItem("Ignore Self Matches");

    ignore_self_match_item.addItemListener(new ItemListener() 
    {
      public void itemStateChanged(ItemEvent event)
      {
        ignore_self_match_flag = ignore_self_match_item.getState();
        repaint();
      }
    });

    ignore_self_match_item.setState(ignore_self_match_flag);

    popup.add(ignore_self_match_item);

    add(popup);
    popup.show(this, event.getX(), event.getY());
  }


  private ColorChooserShades createColours(String title, Color initialColour) 
  {
    //Make sure we have nice window decorations.
    JFrame.setDefaultLookAndFeelDecorated(true);

    //Create and set up the window.
    JFrame frame = new JFrame("ColorChooserDemo");
    frame.setDefaultCloseOperation(JFrame.EXIT_ON_CLOSE);

    //Create and set up the content pane.
    ColorChooserShades newContentPane = new ColorChooserShades(title,initialColour);

    Object[] possibleValues = { "OK", "CANCEL" };
    int select = JOptionPane.showOptionDialog(null, newContentPane,
                                 "Colour Selection",
                                 JOptionPane.DEFAULT_OPTION, 
                                 JOptionPane.QUESTION_MESSAGE,null,
                                 possibleValues, possibleValues[0]);
    if(select == 0)
      return newContentPane;

    return null;
  }


  /**
   *  Handle a mouse press event on the drawing canvas - select on click,
   *  select and broadcast it on double click.
   **/
  private void handleCanvasMousePress(final MouseEvent event) 
  {
    if(event.getID() != MouseEvent.MOUSE_PRESSED) 
      return;

    if(event.getClickCount() == 2) 
      handleCanvasDoubleClick(event);
    else 
      handleCanvasSingleClick(event);

    repaint();
  }

  /**
   *  Handle a double click on the canvas.
   **/
  private void handleCanvasDoubleClick(final MouseEvent event) 
  {
    // there should be only one match in the array
    if(selected_matches != null) 
      alignAt(selected_matches.elementAt(0));
  }

  /**
   *  Send an AlignmentEvent to all the AlignmentListeners.
   *  @param align_match The AlignMatch that we have just centred on.
   **/
  public void alignAt(final AlignMatch align_match) 
  {
    final java.util.Vector targets;
    // copied from a book - synchronizing the whole method might cause a
    // deadlock
    synchronized(this) 
    {
      targets = (java.util.Vector)alignment_event_listeners.clone();
    }

    for(int i = 0; i < targets.size(); ++i) 
    {
      final AlignmentListener listener =
        (AlignmentListener)targets.elementAt(i);

      listener.alignMatchChosen(new AlignmentEvent(align_match));
    }
  }

  /**
   *  Handle a single click on the canvas.
   **/
  private void handleCanvasSingleClick(final MouseEvent event) 
  {
    if(modifiersForLockToggle(event)) 
      toggleDisplayLock();
    else 
    {
      if(!event.isShiftDown()) 
        selected_matches = null;
      
      toggleSelection(event.getPoint());
    }
  }

  /**
   *  Add or remove the match at the given mouse position to the selection.
   **/
  private void toggleSelection(final Point point) 
  {
    final AlignMatch clicked_align_match =
      getAlignMatchFromPosition(point);

    if(clicked_align_match != null) 
    {
      if(selected_matches == null) 
      {
        selected_matches = new AlignMatchVector ();
        selected_matches.add (clicked_align_match);
      } 
      else 
      {
        if(selected_matches.contains(clicked_align_match)) 
        {
          selected_matches.remove(clicked_align_match);
          if(selected_matches.size() == 0)
            selected_matches = null;
        }
        else
          selected_matches.add(clicked_align_match);
      }
    }

    selectionChanged();
  }

  /**
   *  Return the AlignMatch at the given Point on screen or null if there is
   *  no match at that point.  The alignment_data_array is searched in reverse
   *  order.
   **/
  private AlignMatch getAlignMatchFromPosition(final Point click_point) 
  {
    final int canvas_height = getSize().height;
    final int canvas_width  = getSize().width;

    final int subject_length = getSubjectForwardStrand().getSequenceLength();
    final int query_length   = getQueryForwardStrand().getSequenceLength();

    final boolean subject_flipped = subjectIsRevComp();
    final boolean query_flipped   = queryIsRevComp();

    final int all_matches_length = all_matches.length;
    final float base_width       = last_subject_event.getBaseWidth();
    final float query_base_width = last_query_event.getBaseWidth();
    
    final int subject_start = last_subject_event.getStart();
    final int query_start   = last_query_event.getStart();
    final boolean subject_is_rev_comp = subjectIsRevComp();
    final boolean query_is_rev_comp   = queryIsRevComp();
    boolean is_rev_match;
    int[] match_x_positions;

    for(int i = all_matches_length - 1; i >= 0 ; --i) 
    {
      final AlignMatch this_match = all_matches [i];

      is_rev_match = this_match.isRevMatch();
      match_x_positions =
        getMatchCoords(canvas_width, this_match, subject_length, query_length,
                       subject_flipped, query_flipped, base_width, query_base_width, subject_start, 
                       query_start, subject_is_rev_comp, query_is_rev_comp, is_rev_match);

      if(match_x_positions == null) 
        continue;

      if(!isVisible(this_match)) 
        continue;

      final int subject_start_x = match_x_positions[0];
      final int subject_end_x   = match_x_positions[1];
      final int query_start_x   = match_x_positions[2];
      final int query_end_x     = match_x_positions[3];

      // this is the x coordinate of the point where the line y = click_point
      // hits the left edge of the match box
      final double match_left_x =
        subject_start_x +
        (1.0 * (query_start_x - subject_start_x)) *
        (1.0 * click_point.y / canvas_height);

      // this is the x coordinate of the point where the line y = click_point
      // hits the right edge of the match box
      final double match_right_x =
        subject_end_x +
        (1.0 * (query_end_x - subject_end_x)) *
        (1.0 * click_point.y / canvas_height);

      if(click_point.x >= match_left_x - 1 &&
         click_point.x <= match_right_x + 1 ||
         click_point.x <= match_left_x + 1 &&
         click_point.x >= match_right_x - 1) 
        return this_match;
    }

    return null;
  }