Newer
Older
<SECT1 ID="FILEMENU">
<TITLE>The File Menu</TITLE>
<PARA>
</PARA>
<SECT2 ID="FILEMENU-READ-AN-ENTRY">
<TITLE>Read An Entry ...</TITLE>
<PARA>
Read an entry for this sequence, but keep it separate from the others. See
<XREF LINKEND="CONCEPTS-ENTRY"> to see what we mean when we say "entry". The
new entry will be marked as active (see <XREF LINKEND="CONCEPTS-ACTIVEENTRY">)
and will be the new default entry (see <XREF
LINKEND="CONCEPTS-DEFAULTENTRY">).
</PARA>
<PARA>
This function only reads the feature section of the input file - the sequence
(if any) is ignored.
</PARA>
<PARA>
&prog; can read these feature files formats:
</PARA>
<ITEMIZEDLIST>
<LISTITEM>
<PARA>
<ULINK URL="http://www.ebi.ac.uk/embl/Documentation/FT_definitions/feature_table.html">EMBL or GenBank feature tables</ULINK>
</PARA>
</LISTITEM>
<LISTITEM>
<PARA>
<ULINK URL="http://www.sequenceontology.org/resources/gff3.html">GFF files</ULINK>
</PARA>
</LISTITEM>
<LISTITEM>
<PARA>
The output of <ULINK
URL="http://www.cgr.ki.se/cgr/groups/sonnhammer/MSPcrunch.html"><COMMAND>MSPcrunch</COMMAND></ULINK>.
<COMMAND>MSPcrunch</COMMAND> must be run with the <COMMAND>-x</COMMAND> or
<COMMAND>-d</COMMAND> flags.
</PARA>
</LISTITEM>
<LISTITEM>
<PARA>
The output of <ULINK URL="http://www.ncbi.nlm.nih.gov/BLAST/">blastall</ULINK>
version 2.2.2 or better. <COMMAND>blastall</COMMAND> must be run with the
<COMMAND>-m 8</COMMAND> flag which generates one line of information per HSP.
rather than displaying each BLAST hit as a feature.
</PARA>
</LISTITEM>
</ITEMIZEDLIST>
</SECT2>
<SECT2 ID="FILEMENU-SAVE-ENTRY">
<TITLE>Save Entry</TITLE>
<PARA>
Save the entry to the file it came from, unless the entry has been
given a new name, in which case the entry is saved to a file with that name.
If the entry has no name, &prog; will prompt the user for a new name.
</PARA>
</SECT2>
<SECT2 ID="FILEMENU-SAVE-ALL">
<TITLE>Save All</TITLE>
<PARA>
Save all the entries that have been loaded on top of this sequence.
</PARA>
</SECT2>
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
<SECT2 ID="WRITEMENU">
<TITLE>Write</TITLE>
<PARA>
</PARA>
<SECT3 ID="WRITEMENU-AMINO-ACIDS-OF-SELECTED-FEATURES">
<TITLE>Amino Acids Of Selected Features</TITLE>
<PARA>
Prompt for a file name and then write the translation of the bases of the
selected features to that file. The file is written in FASTA format.
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-PIR-DATABASE-OF-SELECTED-FEATURES">
<TITLE>PIR Database Of Selected Features</TITLE>
<PARA>
Prompt for a file name and then write the translation of the bases of the
selected features to that file. The file is written in PIR format (similar to
FASTA, but with a * as the last line of each record).
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-BASES-OF-SELECTION">
<TITLE>Bases Of Selection</TITLE>
<PARA>
Prompt for a file name and then write the bases of the selection to that
file in the selected format. If the selection consists of features (rather
than a base range) then the bases of each feature
will be written to the file as a separate record. If the selection is a range
of bases, then those bases will be written.
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-UPSTREAM-BASES-OF-SELECTION">
<TITLE>Upstream Bases Of Selection</TITLE>
<PARA>
Prompt for a number and a file name, then write that many bases upstream of
each selected feature to the file in the selected format. For example if the
selected feature has a
location of "<LITERAL>100..200</LITERAL>", then asking for 50 upstream will
write the bases in the range 50 to 99. Writing upstream bases of a feature on
the complementary strand will work in the expected way.
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-DOWNSTREAM-BASES-OF-SELECTION">
<TITLE>Downstream Bases Of Selection</TITLE>
<PARA>
Prompt for a number and a file name, then write that many bases downstream of
each selected feature to the file in the selected format.
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-ALL-BASES">
<TITLE>All Bases</TITLE>
<PARA>
Prompt for a file name, then write the complete sequence to that file in the
selected format.
</PARA>
</SECT3>
<SECT3 ID="WRITEMENU-CODON-USAGE">
<TITLE>Codon Usage of Selected Features</TITLE>
<PARA>
Prompt for a file name, then write a codon usage table for the selected
features. The file in written in the same format as the data at <ULINK
URL="http://www.kazusa.or.jp/codon/" TYPE="external">Kazusa codon usage
database site</ULINK>. In the output file each codon is followed by it's
occurrence count (per thousand) and it's percentage occurrence. (See <XREF
LINKEND="GRAPHMENU-USAGE-PLOTS"> to find out how to plot a usage graph).
</PARA>
</SECT3>
</SECT2>
<SECT2 ID="FILEMENU-EDIT-IN-ARTEMIS">
<TITLE>Edit In Artemis ...</TITLE>
<PARA>
Open an &art; edit window for this sequence and features. All changes
made in the &art; window will be immediately visible in the &prog; window and
vice versa.
</PARA>
<PARA>
See <ULINK URL="ftp://ftp.sanger.ac.uk/pub4/resources/software/artemis/artemis.pdf">the