Newer
Older
laststart = -1;
repaint();
}
}
});
JMenuItem readList = new JMenuItem("List Reads ...");
menu.add(readList);
readList.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final JMenuItem bamSplitter = new JMenuItem("Clone window");
bamSplitter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
openBamView(new Vector<String>(bamList));
}
});
menu.add(new JSeparator());
menu.add(bamSplitter);
//
JMenu coverageMenu = new JMenu("Coverage Options");
coverageView.init(this, 0.f, 0, 0);
coverageView.createMenus(coverageMenu);
viewMenu.add(new JSeparator());
viewMenu.add(coverageMenu);
private ReadGroupFrame getReadGroupFrame()
{
if(readGrpFrame == null)
readGrpFrame = new ReadGroupFrame(readGroups, BamView.this);
return readGrpFrame;
}
private JComponent bamTopPanel(final JFrame frame)
{
final JComponent topPanel;
{
topPanel = new JPanel(new FlowLayout(FlowLayout.LEADING, 0, 0));
if(feature_display != null)
this.selection = feature_display.getSelection();
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
JMenu fileMenu = new JMenu("File");
topPanel.add(fileMenu);
JMenuItem readBam = new JMenuItem("Open new BamView ...");
fileMenu.add(readBam);
readBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
JMenuItem saveAs = new JMenuItem("Save As Image File (png/jpeg/svg) ...");
fileMenu.add(saveAs);
saveAs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
PrintBamView.print((JPanel)mainPanel.getParent());
}
});
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JMenuItem close = new JMenuItem("Close");
fileMenu.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView.this.setVisible(false);
Component comp = BamView.this;
while( !(comp instanceof JFrame) )
comp = comp.getParent();
((JFrame)comp).dispose();
}
});
JMenuItem exit = new JMenuItem("Exit");
fileMenu.add(new JSeparator());
fileMenu.add(exit);
exit.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
int status = JOptionPane.showConfirmDialog(BamView.this,
"Exit BamView?", "Exit",
JOptionPane.OK_CANCEL_OPTION);
if(status != JOptionPane.OK_OPTION)
return;
System.exit(0);
}
});
addKeyListener(new KeyAdapter()
{
public void keyPressed(final KeyEvent event)
{
switch (event.getKeyCode())
{
case KeyEvent.VK_UP:
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
break;
case KeyEvent.VK_DOWN:
if (showBaseAlignment)
break;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
break;
default:
break;
}
}
});
}
if(seqNames.size() > 1)
{
int len = 0;
for(int i=0; i<seqNames.size(); i++)
len += seqLengths.get(seqNames.get(i));
if(feature_display != null &&
len == feature_display.getSequenceLength())
concatSequences = true;
else if(bases != null &&
len == bases.getLength() )
concatSequences = true;
}
// auto hide top panel
final JCheckBox buttonAutoHide = new JCheckBox("Hide", (frame == null));
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buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
{
public void mouseDragged(MouseEvent event)
{
handleCanvasMouseDrag(event);
}
public void mouseMoved(MouseEvent e)
{
lastMousePoint = e.getPoint();
int thisHgt = HEIGHT;
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
if (y < thisHgt)
{
topPanel.setVisible(true);
}
else
{
if (buttonAutoHide.isSelected())
topPanel.setVisible(false);
}
mainPanel.repaint();
mainPanel.revalidate();
}
};
addMouseMotionListener(mouseMotionListener);
combo = new SequenceComboBox(seqNames){
private static final long serialVersionUID = 1L;
public void update(IndexReferenceEvent event)
{
laststart = -1;
if(feature_display != null)
setZoomLevel(feature_display.getMaxVisibleBases());
else
setZoomLevel(BamView.this.nbasesInView);
}
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topPanel.add(combo);
if(feature_display == null)
{
final JTextField baseText = new JTextField(8);
JButton goTo = new JButton("GoTo:");
goTo.setToolTipText("Go to base position");
goTo.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
try
{
int basePosition = Integer.parseInt(baseText.getText());
scrollBar.setValue(basePosition);
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(BamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
}
});
topPanel.add(goTo);
topPanel.add(baseText);
JButton zoomIn = new JButton("-");
zoomIn.setToolTipText("Zoom out (up arrow)");
Insets ins = new Insets(1,1,1,1);
zoomIn.setMargin(ins);
zoomIn.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
}
});
topPanel.add(zoomIn);
JButton zoomOut = new JButton("+");
zoomOut.setToolTipText("Zoom in (down arrow)");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
}
});
topPanel.add(zoomOut);
}
topPanel.add(buttonAutoHide);
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if(feature_display != null)
{
JButton close = new JButton("Close");
topPanel.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final JPanel containerPanel = (JPanel) mainPanel.getParent();
feature_display.removeDisplayAdjustmentListener(BamView.this);
feature_display.getSelection().removeSelectionChangeListener(BamView.this);
containerPanel.remove(mainPanel);
if(containerPanel.getComponentCount() > 0)
containerPanel.revalidate();
else
{
if(entry_edit != null)
entry_edit.setNGDivider();
else
containerPanel.setVisible(false);
}
}
});
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
private void setViewportMidPoint()
{
Point p = jspView.getViewport().getLocation();
p.y = (getHeight() - jspView.getViewport().getViewRect().height)/2;
jspView.getViewport().setViewPosition(p);
}
private void setViewportBtm()
{
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
}
if(feature_display != null)
return feature_display.getForwardBaseAtLeftEdge();
else
return scrollBar.getValue();
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
if(isBaseAlignmentView(pixPerBase))
this.nbasesInView = (int)(mainPanel.getWidth()/pixPerBase);
if(ruler == null)
ruler = new Ruler();
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
markInsertions.setEnabled(true);
if(!isCoverageView(pixPerBase) && nbasesInView >= MAX_BASES)
{
cbLastSelected = getSelectedCheckBoxMenuItem();
cbCoverageView.setSelected(true);
coverageView.setPlotByStrand(false);
else if(isCoverageView(pixPerBase) && nbasesInView < MAX_BASES && cbLastSelected != null)
{
cbLastSelected.setSelected(true);
cbLastSelected = null;
}
else
setViewportMidPoint();
markInsertions.setEnabled(false);
{
scrollBar.setValues(startValue, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.setBlockIncrement(nbasesInView);
}
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}
/**
* Return an Artemis entry from a file
* @param entryFileName
* @param entryGroup
* @return
* @throws NoSequenceException
*/
private Entry getEntry(final String entryFileName, final EntryGroup entryGroup)
throws NoSequenceException
{
final Document entry_document = DocumentFactory.makeDocument(entryFileName);
final EntryInformation artemis_entry_information =
Options.getArtemisEntryInformation();
//System.out.println(entryFileName);
final uk.ac.sanger.artemis.io.Entry new_embl_entry =
EntryFileDialog.getEntryFromFile(null, entry_document,
artemis_entry_information,
false);
if(new_embl_entry == null) // the read failed
return null;
Entry entry = null;
try
{
if(entryGroup.getSequenceEntry() != null)
bases = entryGroup.getSequenceEntry().getBases();
else
entry = new Entry(bases,new_embl_entry);
entryGroup.add(entry);
}
catch(OutOfRangeException e)
{
new MessageDialog(null, "read failed: one of the features in " +
entryFileName + " has an out of range " +
"location: " + e.getMessage());
}
return entry;
}
private boolean isShowScale()
{
return (feature_display == null ? true : false);
}
public JScrollPane getJspView()
{
return jspView;
}
/**
* Handle a mouse drag event on the drawing canvas.
**/
if(event.getButton() == MouseEvent.BUTTON3 || bases == null)
tjc
committed
highlightSAMRecord = null;
if(event.getClickCount() > 1)
{
getSelection().clear();
repaint();
return;
}
highlightRange(event,
MouseEvent.BUTTON1_DOWN_MASK & MouseEvent.BUTTON2_DOWN_MASK);
}
/**
*
* @param event
* @param onmask
*/
protected void highlightRange(MouseEvent event, int onmask)
int start = (int) ( ( (event.getPoint().getX())/pixPerBase) + getBaseAtStartOfView() );
if(start < 1)
start = 1;
if(start > seqLength)
start = seqLength;
if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
dragStart = start;
else if((event.getModifiersEx() & onmask) != onmask)
dragStart = -1;
if(dragStart < 0)
drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
else
drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
getSelection().setMarkerRange(drag_range);
repaint();
/**
* Get the colour for the given read given to it by the coverage plot.
* @param samRecord
* @return
*/
private Color getColourByCoverageColour(BamViewRecord samRecord)
short fileIndex = 0;
fileIndex = samRecord.bamIndex;
return getColourByCoverageColour(fileIndex);
}
private Color getColourByCoverageColour(final short fileIndex)
{
LineAttributes lines[] = CoveragePanel.getLineAttributes(bamList.size());
return lines[fileIndex].getLineColour();
}
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protected int getMaxBases()
{
return MAX_BASES;
}
protected void setMaxBases(int max)
{
MAX_BASES = max;
}
private boolean isStackView()
{
return cbStackView.isSelected();
}
private boolean isPairedStackView()
{
return cbPairedStackView.isSelected();
}
private boolean isStrandStackView()
{
return cbStrandStackView.isSelected();
}
private boolean isCoverageView(float pixPerBase)
{
if(isBaseAlignmentView(pixPerBase))
return false;
return cbCoverageView.isSelected() || cbCoverageStrandView.isSelected() || cbCoverageHeatMap.isSelected();
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}
private boolean isIsizeStackView()
{
return cbIsizeStackView.isSelected();
}
private boolean isBaseAlignmentView(float pixPerBase)
{
if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
return true;
return false;
}
private JCheckBoxMenuItem getSelectedCheckBoxMenuItem()
{
if(isStackView())
return cbStackView;
if(isPairedStackView())
return cbPairedStackView;
if(isStrandStackView())
return cbStrandStackView;
if(isIsizeStackView())
return cbIsizeStackView;
if(cbCoverageView.isSelected())
return cbCoverageView;
if(cbCoverageHeatMap.isSelected())
return cbCoverageHeatMap;
protected List<BamViewRecord> getReadsInView()
protected int getBasesInView()
{
return nbasesInView;
}
protected void setHighlightSAMRecord(BamViewRecord highlightSAMRecord)
{
this.highlightSAMRecord = highlightSAMRecord;
}
protected BamViewRecord getHighlightSAMRecord()
protected FeatureDisplay getFeatureDisplay()
{
return feature_display;
}
/**
* @return the combo
*/
public SequenceComboBox getCombo()
{
return combo;
}
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private String getVersion()
{
final ClassLoader cl = this.getClass().getClassLoader();
try
{
String line;
InputStream in = cl.getResourceAsStream("etc/versions");
BufferedReader reader = new BufferedReader(new InputStreamReader(in));
while((line = reader.readLine()) != null)
{
if(line.startsWith("BamView"))
return line.substring( "BamView".length() ).trim();
}
reader.close();
in.close();
}
catch (Exception ex)
{
}
return null;
}
public void openBamView(final List<String> bamsList)
BamView bamView = new BamView(bamsList,
null, nbasesInView, entry_edit,
feature_display, bases, (JPanel) mainPanel.getParent(), null);
bamView.getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
if(feature_display != null)
{
feature_display.addDisplayAdjustmentListener(bamView);
feature_display.getSelection().addSelectionChangeListener(bamView);
if(entry_edit != null)
entry_edit.getOneLinePerEntryDisplay().addDisplayAdjustmentListener(bamView);
if(feature_display.getEntryGroup().getSequenceEntry().getEMBLEntry().getSequence()
instanceof uk.ac.sanger.artemis.io.IndexFastaStream)
{
{
// add reference sequence selection listeners
entry_edit.getEntryGroupDisplay().getIndexFastaCombo().addIndexReferenceListener(bamView.getCombo());
bamView.getCombo().addIndexReferenceListener(entry_edit.getEntryGroupDisplay().getIndexFastaCombo());
public void displayAdjustmentValueChanged(final DisplayAdjustmentEvent event)
if(event.getType() == DisplayAdjustmentEvent.REV_COMP_EVENT &&
event.isRevCompDisplay())
JOptionPane.showMessageDialog(this,
"Flipping the display is not supported by BamView.", "Warning",
JOptionPane.WARNING_MESSAGE);
if(!asynchronous)
{
// if not asynchronous
displayAdjustmentWork(event);
return;
}
public Object construct()
{
try
{
Thread.sleep(500);
}
catch (InterruptedException e)
{
e.printStackTrace();
}
if(event.getStart() != ((FeatureDisplay)event.getSource()).getForwardBaseAtLeftEdge())
{
waitingFrame.showWaiting("waiting...", mainPanel);
waitingFrame.setVisible(false);
return null;
}
};
worker.start();
/**
* Carry out the display agjustment event action.
* @param event
*/
private void displayAdjustmentWork(final DisplayAdjustmentEvent event)
{
if(event.getType() == DisplayAdjustmentEvent.SCALE_ADJUST_EVENT)
{
laststart = -1;
BamView.this.startBase = event.getStart();
BamView.this.endBase = event.getEnd();
int width = feature_display.getMaxVisibleBases();
setZoomLevel(width);
repaint();
}
else
{
setDisplay(event.getStart(),
event.getStart()+feature_display.getMaxVisibleBases(), event);
repaint();
}
}
public void selectionChanged(SelectionChangeEvent event)
{
repaint();
protected int start;
protected int end;
protected String refSeq;
setPreferredSize(new Dimension(mainPanel.getWidth(), 26));
public void paintComponent(Graphics g)
{
super.paintComponent(g);
Graphics2D g2 = (Graphics2D)g;
drawBaseScale(g2, start, end, 12);
}
private void drawBaseScale(Graphics2D g2, int start, int end, int ypos)
{
int startMark = (((int)(start/10))*10)+1;
if(end > getSequenceLength())
end = getSequenceLength();
for(int i=startMark; i<end; i+=20)
for(int i=startMark; i<end; i+=10)
{
int xpos = (i-start)*ALIGNMENT_PIX_PER_BASE;
xpos+=(ALIGNMENT_PIX_PER_BASE/2);
g2.drawLine(xpos, ypos+1, xpos, ypos+5);
}
if(refSeq != null)
{
ypos+=15;
g2.setColor(LIGHT_GREY);
g2.fillRect(0, ypos-11, getWidth(), 11);
g2.translate(0, 16);
drawSelectionRange(g2, ALIGNMENT_PIX_PER_BASE, start, end, Color.yellow);
g2.translate(0, -16);
g2.setColor(Color.black);
g2.drawString(refSeq, 0, ypos-2);
}
/**
* Popup menu listener
*/
class PopupListener extends MouseAdapter
{
private JMenuItem gotoMateMenuItem;
private JMenuItem showDetails;
private JMenu coverageMenu;
private JMenuItem createGroup;
tjc
committed
public void mouseClicked(MouseEvent e)
{
if(e.isPopupTrigger() ||
e.getButton() == MouseEvent.BUTTON3)
return;
BamView.this.requestFocus();
if(e.getClickCount() > 1)
getSelection().clear();
else if(e.getButton() == MouseEvent.BUTTON1)
{
if(isCoverageView(getPixPerBaseByWidth()))
coverageView.singleClick(e.isShiftDown(),
e.getPoint().y-getJspView().getViewport().getViewPosition().y);
else
highlightSAMRecord = mouseOverSAMRecord;
}
else
highlightRange(e, MouseEvent.BUTTON2_DOWN_MASK);
repaint();
}
public void mousePressed(MouseEvent e)
{
maybeShowPopup(e);
}
public void mouseReleased(MouseEvent e)
{
maybeShowPopup(e);
}
private void maybeShowPopup(MouseEvent e)
{
if(e.isPopupTrigger())
//
// coverage heatmap menu options
if(coverageMenu != null)
popup.remove(coverageMenu);
if(isCoverageView(getPixPerBaseByWidth()) && coverageView.isPlotHeatMap())
{
if(coverageMenu == null)
{
coverageMenu = new JMenu("Coverage HeatMap");
final JCheckBoxMenuItem coverageGrid = new JCheckBoxMenuItem("Show heatmap grid", false);
coverageGrid.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
coverageView.showLabels(coverageGrid.isSelected());
}
});
coverageMenu.add(coverageGrid);
createGroup = new JMenuItem("Create group from selected BAMs");
createGroup.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
String groupName = "group_"+n;
groupsFrame.addGroup(groupName);
final List<String> selected = coverageView.getSelected();
for(String sel: selected)
groupsFrame.addToGroup((new File(sel)).getName(), groupName);
groupsFrame.updateAndDisplay();
n++;
}
});
coverageMenu.add(createGroup);
}
createGroup.setEnabled(coverageView.hasSelectedBams());
popup.add(coverageMenu);
}
if(gotoMateMenuItem != null)
popup.remove(gotoMateMenuItem);
tjc
committed
if(showDetails != null)
popup.remove(showDetails);
if( mouseOverSAMRecord != null &&
mouseOverSAMRecord.sam.getReadPairedFlag() &&
!mouseOverSAMRecord.sam.getMateUnmappedFlag() )
{
final BamViewRecord thisSAMRecord = mouseOverSAMRecord;
gotoMateMenuItem = new JMenuItem("Go to mate of : "+
thisSAMRecord.sam.getReadName());
gotoMateMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
String name = thisSAMRecord.sam.getMateReferenceName();
if(name.equals("="))
name = thisSAMRecord.sam.getReferenceName();
int offset = getSequenceOffset(name);
if(feature_display != null)
feature_display.makeBaseVisible(
thisSAMRecord.sam.getMateAlignmentStart()+offset);
else
scrollBar.setValue(
thisSAMRecord.sam.getMateAlignmentStart()+offset-
(nbasesInView/2));
highlightSAMRecord = thisSAMRecord;
}
});
popup.add(gotoMateMenuItem);
tjc
committed
}
if( mouseOverSAMRecord != null)
{
final BamViewRecord thisSAMRecord = mouseOverSAMRecord;
tjc
committed
showDetails = new JMenuItem("Show details of : "+
thisSAMRecord.sam.getReadName());
tjc
committed
showDetails.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
openFileViewer(thisSAMRecord.sam, getMate(thisSAMRecord), bamList);
tjc
committed
});
popup.add(showDetails);
}
popup.show(e.getComponent(),
e.getX(), e.getY());
protected static void openFileViewer(SAMRecord readRecord, SAMRecord mateRecord, List<String> bamList)
{
FileViewer viewDetail = new FileViewer(readRecord.getReadName(), true, false, false);
appendToDetailView(readRecord, mateRecord, viewDetail, bamList);
private static void appendToDetailView(final SAMRecord sam,
final SAMRecord mate,
final FileViewer viewer,
final List<String> bamList)
tjc
committed
{
if(bamList.size() > 1 && sam.getAttribute("FL") != null)
int bamIdx = (Integer)sam.getAttribute("FL");
if(bamIdx < bamList.size())
viewer.appendString("File "+bamList.get(bamIdx)+"\n\n", Level.INFO);
viewer.appendString("Read Name "+sam.getReadName()+"\n", Level.INFO);
viewer.appendString("Coordinates "+sam.getAlignmentStart()+".."+
sam.getAlignmentEnd()+"\n", Level.DEBUG);
if(sam.getReadGroup() != null)
viewer.appendString("Read Group "+sam.getReadGroup().getId()+"\n", Level.DEBUG);
viewer.appendString("Length "+sam.getReadLength()+"\n", Level.DEBUG);
viewer.appendString("Reference Name "+sam.getReferenceName()+"\n", Level.DEBUG);