Newer
Older
JMenuItem filter = new JMenuItem("Filter Reads ...");
menu.add(filter);
filter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(filterFrame == null)
filterFrame = new SAMRecordFilter(BamView.this);
else
filterFrame.setVisible(true);
}
});
3014
3015
3016
3017
3018
3019
3020
3021
3022
3023
3024
3025
3026
3027
3028
3029
3030
3031
3032
3033
3034
3035
JMenuItem maxReadCoverage = new JMenuItem("Read Coverage Threshold ...");
menu.add(maxReadCoverage);
maxReadCoverage.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final TextFieldInt maxRead = new TextFieldInt();
maxRead.setValue(MAX_COVERAGE);
int status = JOptionPane.showConfirmDialog(null, maxRead,
"Read Coverage Threshold", JOptionPane.OK_CANCEL_OPTION);
if(status == JOptionPane.OK_OPTION &&
maxRead.getValue() != MAX_COVERAGE)
{
MAX_COVERAGE = maxRead.getValue();
if(MAX_COVERAGE < 1)
MAX_COVERAGE = Integer.MAX_VALUE;
laststart = -1;
repaint();
}
}
});
JMenuItem readList = new JMenuItem("List Reads ...");
menu.add(readList);
readList.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
final JMenuItem bamSplitter = new JMenuItem("Clone window");
bamSplitter.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
openBamView(new Vector<String>(bamList));
}
});
menu.add(new JSeparator());
menu.add(bamSplitter);
//
JMenu coverageMenu = new JMenu("Coverage Options");
coverageView.init(this, 0.f, 0, 0);
coverageView.createMenus(coverageMenu);
viewMenu.add(new JSeparator());
viewMenu.add(coverageMenu);
readGrpFrame = new ReadGroupsFrame(readGroups, BamView.this);
private JComponent bamTopPanel(final JFrame frame)
{
final JComponent topPanel;
{
topPanel = new JPanel(new FlowLayout(FlowLayout.LEADING, 0, 0));
if(feature_display != null)
this.selection = feature_display.getSelection();
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
JMenu fileMenu = new JMenu("File");
topPanel.add(fileMenu);
JMenuItem readBam = new JMenuItem("Open new BamView ...");
fileMenu.add(readBam);
readBam.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
JMenuItem saveAs = new JMenuItem("Save As Image File (png/jpeg/svg) ...");
fileMenu.add(saveAs);
saveAs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
PrintBamView.print((JPanel)mainPanel.getParent());
}
});
3112
3113
3114
3115
3116
3117
3118
3119
3120
3121
3122
3123
3124
3125
3126
3127
3128
3129
3130
3131
3132
3133
3134
3135
3136
3137
3138
3139
3140
3141
3142
3143
3144
3145
3146
3147
3148
3149
3150
3151
3152
3153
3154
3155
3156
3157
3158
3159
3160
3161
3162
3163
3164
3165
3166
3167
3168
3169
3170
3171
3172
3173
3174
3175
3176
3177
3178
JMenuItem close = new JMenuItem("Close");
fileMenu.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
BamView.this.setVisible(false);
Component comp = BamView.this;
while( !(comp instanceof JFrame) )
comp = comp.getParent();
((JFrame)comp).dispose();
}
});
JMenuItem exit = new JMenuItem("Exit");
fileMenu.add(new JSeparator());
fileMenu.add(exit);
exit.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
int status = JOptionPane.showConfirmDialog(BamView.this,
"Exit BamView?", "Exit",
JOptionPane.OK_CANCEL_OPTION);
if(status != JOptionPane.OK_OPTION)
return;
System.exit(0);
}
});
addKeyListener(new KeyAdapter()
{
public void keyPressed(final KeyEvent event)
{
switch (event.getKeyCode())
{
case KeyEvent.VK_UP:
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
break;
case KeyEvent.VK_DOWN:
if (showBaseAlignment)
break;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
break;
default:
break;
}
}
});
}
if(seqNames.size() > 1)
{
int len = 0;
for(int i=0; i<seqNames.size(); i++)
len += seqLengths.get(seqNames.get(i));
if(feature_display != null &&
len == feature_display.getSequenceLength())
concatSequences = true;
else if(bases != null &&
len == bases.getLength() )
concatSequences = true;
}
// auto hide top panel
final JCheckBox buttonAutoHide = new JCheckBox("Hide", (frame == null));
buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
{
public void mouseDragged(MouseEvent event)
{
handleCanvasMouseDrag(event);
}
public void mouseMoved(MouseEvent e)
{
lastMousePoint = e.getPoint();
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
Point p = jspView.getViewport().getViewPosition();
boolean isVis = topPanel.isVisible();
if (y < thisHgt)
{
topPanel.setVisible(true);
}
else
{
if (buttonAutoHide.isSelected())
}
};
addMouseMotionListener(mouseMotionListener);
combo = new SequenceComboBox(seqNames){
private static final long serialVersionUID = 1L;
public void update(IndexReferenceEvent event)
{
laststart = -1;
if(feature_display != null)
setZoomLevel(feature_display.getMaxVisibleBases());
else
setZoomLevel(BamView.this.nbasesInView);
}
3232
3233
3234
3235
3236
3237
3238
3239
3240
3241
3242
3243
3244
3245
3246
3247
3248
3249
3250
3251
3252
3253
3254
3255
3256
3257
3258
3259
topPanel.add(combo);
if(feature_display == null)
{
final JTextField baseText = new JTextField(8);
JButton goTo = new JButton("GoTo:");
goTo.setToolTipText("Go to base position");
goTo.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
try
{
int basePosition = Integer.parseInt(baseText.getText());
scrollBar.setValue(basePosition);
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(BamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
}
});
topPanel.add(goTo);
topPanel.add(baseText);
JButton zoomIn = new JButton("-");
zoomIn.setToolTipText("Zoom out (up arrow)");
Insets ins = new Insets(1,1,1,1);
zoomIn.setMargin(ins);
zoomIn.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
}
});
topPanel.add(zoomIn);
JButton zoomOut = new JButton("+");
zoomOut.setToolTipText("Zoom in (down arrow)");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
}
});
topPanel.add(zoomOut);
}
topPanel.add(buttonAutoHide);
final JSlider slider = new JSlider(13, 52, (int) (readLnHgt*10));
slider.addChangeListener(new ChangeListener(){
public void stateChanged(ChangeEvent arg0)
{
readLnHgt = (slider.getValue()/10.f);
repaintBamView();
}
});
if(feature_display != null)
{
JButton close = new JButton("Close");
topPanel.add(close);
close.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
int status = JOptionPane.showConfirmDialog(frame,
"Close the BAM panel?", "Close",
JOptionPane.OK_CANCEL_OPTION, JOptionPane.QUESTION_MESSAGE);
if(status == JOptionPane.CANCEL_OPTION)
return;
final JPanel containerPanel = (JPanel) mainPanel.getParent();
feature_display.removeDisplayAdjustmentListener(BamView.this);
feature_display.getSelection().removeSelectionChangeListener(BamView.this);
containerPanel.remove(mainPanel);
if(containerPanel.getComponentCount() > 0)
containerPanel.revalidate();
else
{
if(entry_edit != null)
entry_edit.setNGDivider();
else
containerPanel.setVisible(false);
}
}
});
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
private void setViewportMidPoint()
{
Point p = jspView.getViewport().getLocation();
p.y = (getHeight() - jspView.getViewport().getViewRect().height)/2;
jspView.getViewport().setViewPosition(p);
}
private void setViewportBtm()
{
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
}
if(feature_display != null)
return feature_display.getForwardBaseAtLeftEdge();
else
return scrollBar.getValue();
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
if(isBaseAlignmentView(pixPerBase))
this.nbasesInView = (int)(mainPanel.getWidth()/pixPerBase);
if(ruler == null)
ruler = new Ruler();
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
markInsertions.setEnabled(true);
if(!isCoverageView(pixPerBase) && nbasesInView >= MAX_BASES)
{
cbLastSelected = getSelectedCheckBoxMenuItem();
cbCoverageView.setSelected(true);
coverageView.setPlotByStrand(false);
else if(isCoverageView(pixPerBase) && nbasesInView < MAX_BASES && cbLastSelected != null)
{
cbLastSelected.setSelected(true);
cbLastSelected = null;
}
else
setViewportMidPoint();
markInsertions.setEnabled(false);
{
scrollBar.setValues(startValue, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.setBlockIncrement(nbasesInView);
}
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}
/**
* Return an Artemis entry from a file
* @param entryFileName
* @param entryGroup
* @return
* @throws NoSequenceException
*/
private Entry getEntry(final String entryFileName, final EntryGroup entryGroup)
throws NoSequenceException
{
final Document entry_document = DocumentFactory.makeDocument(entryFileName);
final EntryInformation artemis_entry_information =
Options.getArtemisEntryInformation();
//System.out.println(entryFileName);
final uk.ac.sanger.artemis.io.Entry new_embl_entry =
EntryFileDialog.getEntryFromFile(null, entry_document,
artemis_entry_information,
false);
if(new_embl_entry == null) // the read failed
return null;
Entry entry = null;
try
{
if(entryGroup.getSequenceEntry() != null)
bases = entryGroup.getSequenceEntry().getBases();
else
entry = new Entry(bases,new_embl_entry);
entryGroup.add(entry);
}
catch(OutOfRangeException e)
{
new MessageDialog(null, "read failed: one of the features in " +
entryFileName + " has an out of range " +
"location: " + e.getMessage());
}
return entry;
}
private boolean isShowScale()
{
return (feature_display == null ? true : false);
}
public JScrollPane getJspView()
{
return jspView;
}
/**
* Handle a mouse drag event on the drawing canvas.
**/
if(event.getButton() == MouseEvent.BUTTON3 || bases == null)
tjc
committed
highlightSAMRecord = null;
if(event.getClickCount() > 1)
{
getSelection().clear();
repaint();
return;
}
highlightRange(event,
MouseEvent.BUTTON1_DOWN_MASK & MouseEvent.BUTTON2_DOWN_MASK);
}
/**
*
* @param event
* @param onmask
*/
protected void highlightRange(MouseEvent event, int onmask)
int start = (int) ( ( (event.getPoint().getX())/pixPerBase) + getBaseAtStartOfView() );
if(start < 1)
start = 1;
if(start > seqLength)
start = seqLength;
if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
dragStart = start;
else if((event.getModifiersEx() & onmask) != onmask)
dragStart = -1;
if(dragStart < 0)
drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
else
drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
getSelection().setMarkerRange(drag_range);
repaint();
/**
* Get the colour for the given read given to it by the coverage plot.
* @param samRecord
* @return
*/
private Color getColourByCoverageColour(BamViewRecord samRecord)
short fileIndex = 0;
fileIndex = samRecord.bamIndex;
return getColourByCoverageColour(fileIndex);
}
private Color getColourByCoverageColour(final short fileIndex)
{
LineAttributes lines[] = CoveragePanel.getLineAttributes(bamList.size());
return lines[fileIndex].getLineColour();
}
3592
3593
3594
3595
3596
3597
3598
3599
3600
3601
3602
3603
3604
3605
3606
3607
3608
3609
3610
3611
3612
3613
3614
3615
3616
3617
3618
3619
3620
3621
protected int getMaxBases()
{
return MAX_BASES;
}
protected void setMaxBases(int max)
{
MAX_BASES = max;
}
private boolean isStackView()
{
return cbStackView.isSelected();
}
private boolean isPairedStackView()
{
return cbPairedStackView.isSelected();
}
private boolean isStrandStackView()
{
return cbStrandStackView.isSelected();
}
private boolean isCoverageView(float pixPerBase)
{
if(isBaseAlignmentView(pixPerBase))
return false;
return cbCoverageView.isSelected() || cbCoverageStrandView.isSelected() || cbCoverageHeatMap.isSelected();
3623
3624
3625
3626
3627
3628
3629
3630
3631
3632
3633
3634
3635
3636
3637
3638
3639
3640
3641
3642
3643
3644
3645
3646
}
private boolean isIsizeStackView()
{
return cbIsizeStackView.isSelected();
}
private boolean isBaseAlignmentView(float pixPerBase)
{
if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
return true;
return false;
}
private JCheckBoxMenuItem getSelectedCheckBoxMenuItem()
{
if(isStackView())
return cbStackView;
if(isPairedStackView())
return cbPairedStackView;
if(isStrandStackView())
return cbStrandStackView;
if(isIsizeStackView())
return cbIsizeStackView;
if(cbCoverageView.isSelected())
return cbCoverageView;
if(cbCoverageHeatMap.isSelected())
return cbCoverageHeatMap;
protected List<BamViewRecord> getReadsInView()
protected int getBasesInView()
{
return nbasesInView;
}
protected void setHighlightSAMRecord(BamViewRecord highlightSAMRecord)
{
this.highlightSAMRecord = highlightSAMRecord;
}
protected BamViewRecord getHighlightSAMRecord()
protected FeatureDisplay getFeatureDisplay()
{
return feature_display;
}
/**
* @return the combo
*/
public SequenceComboBox getCombo()
{
return combo;
}
3692
3693
3694
3695
3696
3697
3698
3699
3700
3701
3702
3703
3704
3705
3706
3707
3708
3709
3710
3711
3712
3713
private String getVersion()
{
final ClassLoader cl = this.getClass().getClassLoader();
try
{
String line;
InputStream in = cl.getResourceAsStream("etc/versions");
BufferedReader reader = new BufferedReader(new InputStreamReader(in));
while((line = reader.readLine()) != null)
{
if(line.startsWith("BamView"))
return line.substring( "BamView".length() ).trim();
}
reader.close();
in.close();
}
catch (Exception ex)
{
}
return null;
}
public void openBamView(final List<String> bamsList)
BamView bamView = new BamView(bamsList,
null, nbasesInView, entry_edit,
feature_display, bases, (JPanel) mainPanel.getParent(), null);
bamView.getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
getJspView().getVerticalScrollBar().setValue(
bamView.getJspView().getVerticalScrollBar().getMaximum());
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
if(feature_display != null)
{
feature_display.addDisplayAdjustmentListener(bamView);
feature_display.getSelection().addSelectionChangeListener(bamView);
if(entry_edit != null)
entry_edit.getOneLinePerEntryDisplay().addDisplayAdjustmentListener(bamView);
if(feature_display.getEntryGroup().getSequenceEntry().getEMBLEntry().getSequence()
instanceof uk.ac.sanger.artemis.io.IndexFastaStream)
{
{
// add reference sequence selection listeners
entry_edit.getEntryGroupDisplay().getIndexFastaCombo().addIndexReferenceListener(bamView.getCombo());
bamView.getCombo().addIndexReferenceListener(entry_edit.getEntryGroupDisplay().getIndexFastaCombo());
public void displayAdjustmentValueChanged(final DisplayAdjustmentEvent event)
if(event.getType() == DisplayAdjustmentEvent.REV_COMP_EVENT &&
event.isRevCompDisplay())
JOptionPane.showMessageDialog(this,
"Flipping the display is not supported by BamView.", "Warning",
JOptionPane.WARNING_MESSAGE);
if(!asynchronous)
{
// if not asynchronous
displayAdjustmentWork(event);
return;
}
public Object construct()
{
try
{
Thread.sleep(500);
}
catch (InterruptedException e)
{
e.printStackTrace();
}
if(event.getStart() != ((FeatureDisplay)event.getSource()).getForwardBaseAtLeftEdge())
{
waitingFrame.showWaiting("waiting...", mainPanel);
waitingFrame.setVisible(false);
return null;
}
};
worker.start();
/**
* Carry out the display agjustment event action.
* @param event
*/
private void displayAdjustmentWork(final DisplayAdjustmentEvent event)
{
if(event.getType() == DisplayAdjustmentEvent.SCALE_ADJUST_EVENT)
{
laststart = -1;
BamView.this.startBase = event.getStart();
BamView.this.endBase = event.getEnd();
int width = feature_display.getMaxVisibleBases();
setZoomLevel(width);
repaint();
}
else
{
setDisplay(event.getStart(),
event.getStart()+feature_display.getMaxVisibleBases(), event);
repaint();
}
}
public void selectionChanged(SelectionChangeEvent event)
{
repaint();
protected int start;
protected int end;
protected String refSeq;
setPreferredSize(new Dimension(mainPanel.getWidth(), 26));
public void paintComponent(Graphics g)
{
super.paintComponent(g);
Graphics2D g2 = (Graphics2D)g;
drawBaseScale(g2, start, end, 12);
}
private void drawBaseScale(Graphics2D g2, int start, int end, int ypos)
{
int startMark = (((int)(start/10))*10)+1;
if(end > getSequenceLength())
end = getSequenceLength();
for(int i=startMark; i<end; i+=20)
for(int i=startMark; i<end; i+=10)
{
int xpos = (i-start)*ALIGNMENT_PIX_PER_BASE;
xpos+=(ALIGNMENT_PIX_PER_BASE/2);
g2.drawLine(xpos, ypos+1, xpos, ypos+5);
}
if(refSeq != null)
{
ypos+=15;
g2.setColor(LIGHT_GREY);
g2.fillRect(0, ypos-11, getWidth(), 11);
g2.translate(0, 16);
drawSelectionRange(g2, ALIGNMENT_PIX_PER_BASE, start, end, Color.yellow);
g2.translate(0, -16);
g2.setColor(Color.black);
g2.drawString(refSeq, 0, ypos-2);
}
/**
* Popup menu listener
*/
class PopupListener extends MouseAdapter
{
private JMenuItem gotoMateMenuItem;
private JMenuItem showDetails;
private JMenu coverageMenu;
private JMenuItem createGroup;
tjc
committed
public void mouseClicked(MouseEvent e)
{
if(e.isPopupTrigger() ||
e.getButton() == MouseEvent.BUTTON3)
return;
BamView.this.requestFocus();
if(e.getClickCount() > 1)
getSelection().clear();
else if(e.getButton() == MouseEvent.BUTTON1)
{
if(isCoverageView(getPixPerBaseByWidth()))
coverageView.singleClick(e.isShiftDown(),
e.getPoint().y-getJspView().getViewport().getViewPosition().y);
else
highlightSAMRecord = mouseOverSAMRecord;
}
else
highlightRange(e, MouseEvent.BUTTON2_DOWN_MASK);
repaint();
}
public void mousePressed(MouseEvent e)
{
maybeShowPopup(e);
}
public void mouseReleased(MouseEvent e)
{
maybeShowPopup(e);
}
private void maybeShowPopup(MouseEvent e)
{
if(e.isPopupTrigger())
//
// coverage heatmap menu options
if(coverageMenu != null)
popup.remove(coverageMenu);
if(isCoverageView(getPixPerBaseByWidth()) && coverageView.isPlotHeatMap())
{
if(coverageMenu == null)
{
coverageMenu = new JMenu("Coverage HeatMap");
final JCheckBoxMenuItem coverageGrid = new JCheckBoxMenuItem("Show heatmap grid", false);
coverageGrid.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
coverageView.showLabels(coverageGrid.isSelected());
}
});
coverageMenu.add(coverageGrid);
createGroup = new JMenuItem("Create group from selected BAMs");
createGroup.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
String groupName = "group_"+n;
groupsFrame.addGroup(groupName);
final List<String> selected = coverageView.getSelected();
for(String sel: selected)
groupsFrame.addToGroup((new File(sel)).getName(), groupName);
groupsFrame.updateAndDisplay();
n++;
}
});
coverageMenu.add(createGroup);
}
createGroup.setEnabled(coverageView.hasSelectedBams());
popup.add(coverageMenu);
}
if(gotoMateMenuItem != null)
popup.remove(gotoMateMenuItem);
tjc
committed
if(showDetails != null)
popup.remove(showDetails);
if( mouseOverSAMRecord != null &&
mouseOverSAMRecord.sam.getReadPairedFlag() &&
!mouseOverSAMRecord.sam.getMateUnmappedFlag() )
{
final BamViewRecord thisSAMRecord = mouseOverSAMRecord;
gotoMateMenuItem = new JMenuItem("Go to mate of : "+
thisSAMRecord.sam.getReadName());
gotoMateMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
String name = thisSAMRecord.sam.getMateReferenceName();
if(name.equals("="))
name = thisSAMRecord.sam.getReferenceName();
int offset = getSequenceOffset(name);
if(feature_display != null)
feature_display.makeBaseVisible(
thisSAMRecord.sam.getMateAlignmentStart()+offset);