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if(Math.abs(samRecord.getMateAlignmentStart()-samRecord.getAlignmentEnd())*pixPerBase > 2.f)
{
g2.setStroke(originalStroke);
g2.setColor(Color.LIGHT_GRAY);
if(samRecord.getAlignmentEnd() < samRecord.getMateAlignmentStart())
{
int nextStart =
(int)((samRecord.getMateAlignmentStart()-getBaseAtStartOfView()+offset)*pixPerBase);
drawTranslucentLine(g2,
(int)((thisStart-getBaseAtStartOfView())*pixPerBase), nextStart, ypos);
int nextStart =
(int)((samRecord.getMateAlignmentStart()-getBaseAtStartOfView()+offset)*pixPerBase);
drawTranslucentLine(g2,
(int)((thisEnd-getBaseAtStartOfView())*pixPerBase), nextStart, ypos);
if(offTheTop)
g2.setColor(darkOrange);
else if(samRecord.getReadNegativeStrandFlag()) // strand of the query (1 for reverse)
g2.setColor(Color.red);
else
g2.setColor(Color.blue);
showSNPsOnReads(g2, samRecord, pixPerBase, ypos, offset);
private void drawScale(Graphics2D g2, int start, int end, float pixPerBase)
{
g2.setColor(Color.black);
g2.drawLine( 0, getHeight()-14,
(int)((end - getBaseAtStartOfView())*pixPerBase), getHeight()-14);
int interval = end-start;
if(interval > 256000)
drawTicks(g2, start, end, pixPerBase, 512000);
else if(interval > 64000)
drawTicks(g2, start, end, pixPerBase, 12800);
else if(interval > 16000)
drawTicks(g2, start, end, pixPerBase, 3200);
else if(interval > 4000)
drawTicks(g2, start, end, pixPerBase, 800);
else if(interval > 1000)
drawTicks(g2, start, end, pixPerBase, 400);
drawTicks(g2, start, end, pixPerBase, 100);
}
private void drawTicks(Graphics2D g2, int start, int end, float pixPerBase, int division)
{
int markStart = (Math.round(start/division)*division);
if(markStart < 1)
markStart = 1;
{
x = (sm-getBaseAtStartOfView())*pixPerBase;
g2.drawLine((int)x, getHeight()-14,(int)x, getHeight()-12);
}
x = (m-getBaseAtStartOfView())*pixPerBase;
g2.drawString(Integer.toString(m), x, getHeight()-1);
g2.drawLine((int)x, getHeight()-14,(int)x, getHeight()-11);
{
x = (sm-getBaseAtStartOfView())*pixPerBase;
g2.drawLine((int)x, getHeight()-14,(int)x, getHeight()-12);
}
/**
* Draw a y-scale for inferred size (isize) of reads.
* @param g2
* @param xScaleHeight
*/
private void drawYScale(Graphics2D g2, int xScaleHeight)
int maxY = getPreferredSize().height-xScaleHeight;
for(int i=100; i<maxY; i+=100)
{
int ypos = getHeight()-i-xScaleHeight;
g2.drawLine(0, ypos, 2, ypos);
g2.drawString(Integer.toString(i), 3, ypos);
/**
* Draw a given read.
* @param g2
* @param thisRead
* @param pixPerBase
* @param stroke
* @param ypos
*/
int offset = getSequenceOffset(thisRead.getReferenceName());
int thisStart = thisRead.getAlignmentStart()+offset-getBaseAtStartOfView();
int thisEnd = thisRead.getAlignmentEnd()+offset-getBaseAtStartOfView();
g2.drawLine((int)( thisStart * pixPerBase), ypos,
(int)( thisEnd * pixPerBase), ypos);
// test if the mouse is over this read
if(lastMousePoint != null)
{
if(lastMousePoint.getY()+2 > ypos && lastMousePoint.getY()-2 < ypos)
if(lastMousePoint.getX() > thisStart * pixPerBase &&
lastMousePoint.getX() < thisEnd * pixPerBase)
{
mouseOverSAMRecord = thisRead;
}
}
showSNPsOnReads(g2, thisRead, pixPerBase, ypos, offset);
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/**
* Highlight a selected range
* @param g2
* @param pixPerBase
* @param start
* @param end
*/
private void drawSelectionRange(Graphics2D g2, float pixPerBase, int start, int end)
{
if(getSelection() != null)
{
Range selectedRange = getSelection().getSelectionRange();
if(selectedRange != null)
{
int rangeStart = selectedRange.getStart();
int rangeEnd = selectedRange.getEnd();
if(end < rangeStart || start > rangeEnd)
return;
int x = (int) (pixPerBase*(rangeStart-getBaseAtStartOfView()));
int width = (int) (pixPerBase*(rangeEnd-rangeStart+1));
g2.setColor(Color.pink);
g2.fillRect(x, 0, width, getHeight());
}
}
}
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/**
* Draw a translucent line
* @param g2
* @param start
* @param end
* @param ypos
*/
private void drawTranslucentLine(Graphics2D g2, int start, int end, int ypos)
{
Composite origComposite = g2.getComposite();
g2.setComposite(translucent);
g2.drawLine(start, ypos, end, ypos);
g2.setComposite(origComposite);
}
/**
* Draw a translucent line
* @param g2
* @param start
* @param end
* @param ypos
*/
private void drawTranslucentJointedLine(Graphics2D g2, int start, int end, int ypos)
{
Composite origComposite = g2.getComposite();
g2.setComposite(translucent);
int mid = (int) ((end-start)/2.f)+start;
//g2.drawLine(start, ypos, end, ypos);
g2.drawLine(start, ypos, mid, ypos-5);
g2.drawLine(mid, ypos-5, end, ypos);
g2.setComposite(origComposite);
}
/**
* Display the SNPs for the given read.
* @param g2
* @param thisRead
* @param pixPerBase
* @param ypos
*/
private void showSNPsOnReads(Graphics2D g2, SAMRecord thisRead,
{
int thisStart = thisRead.getAlignmentStart();
int thisEnd = thisRead.getAlignmentEnd();
// use alignment blocks of the contiguous alignment of
// subsets of read bases to a reference sequence
List<AlignmentBlock> blocks = thisRead.getAlignmentBlocks();
new Range(thisStart+offset, thisEnd+offset), Bases.FORWARD);
for(int i=0; i<blocks.size(); i++)
AlignmentBlock block = blocks.get(i);
for(int j=0; j<block.getLength(); j++)
int readPos = block.getReadStart()-1+j;
int refPos = block.getReferenceStart()+j;
if (Character.toUpperCase(refSeq[refPos-thisStart]) != readSeq[readPos])
{
g2.drawLine((int) ((refPos+offset-getBaseAtStartOfView()) * pixPerBase), ypos + 2,
(int) ((refPos+offset-getBaseAtStartOfView()) * pixPerBase), ypos - 2);
g2.setColor(col);
}
catch (OutOfRangeException e)
{
e.printStackTrace();
/**
* Add the alignment view to the supplied <code>JPanel</code> in
* a <code>JScrollPane</code>.
* @param mainPanel panel to add the alignment to
* @param autohide automatically hide the top panel containing the buttons
*/
public void addJamToPanel(final JPanel mainPanel,
final boolean autohide,
final FeatureDisplay feature_display)
if(feature_display != null)
{
this.feature_display = feature_display;
this.selection = feature_display.getSelection();
if(seqNames.size() > 1)
{
int len = 0;
for(int i=0; i<seqNames.size(); i++)
len += seqLengths.get(seqNames.get(i));
if(len == feature_display.getSequenceLength())
concatSequences = true;
}
}
else
{
topPanel = new JMenuBar();
frame.setJMenuBar((JMenuBar)topPanel);
final JCheckBox buttonAutoHide = new JCheckBox("Hide", autohide);
buttonAutoHide.setToolTipText("Auto-Hide");
final MouseMotionListener mouseMotionListener = new MouseMotionListener()
handleCanvasMouseDragOrClick(event);
}
public void mouseMoved(MouseEvent e)
{
int thisHgt = HEIGHT;
if (thisHgt < 5)
thisHgt = 15;
int y = (int) (e.getY() - jspView.getViewport().getViewRect().getY());
if (y < thisHgt)
}
mainPanel.repaint();
mainPanel.revalidate();
}
};
addMouseMotionListener(mouseMotionListener);
combo = new JComboBox(seqNames);
combo.setEditable(false);
combo.addItemListener(new ItemListener()
{
public void itemStateChanged(ItemEvent e)
{
laststart = -1;
lastend = -1;
setZoomLevel(BamView.this.nbasesInView);
goTo.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
try
{
int basePosition = Integer.parseInt(baseText.getText());
}
catch (NumberFormatException nfe)
{
JOptionPane.showMessageDialog(BamView.this,
"Expecting a base number!", "Number Format",
JOptionPane.WARNING_MESSAGE);
}
JButton zoomIn = new JButton("-");
Insets ins = new Insets(1,1,1,1);
zoomIn.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
JButton zoomOut = new JButton("+");
zoomOut.setMargin(ins);
zoomOut.addActionListener(new ActionListener()
public void actionPerformed(ActionEvent e)
{
if (showBaseAlignment)
return;
setZoomLevel((int) (BamView.this.nbasesInView * .9));
jspView = new JScrollPane(this,
JScrollPane.VERTICAL_SCROLLBAR_AS_NEEDED,
setDisplay(1, nbasesInView, null);
mainPanel.setLayout(new BorderLayout());
if(topPanel instanceof JPanel)
mainPanel.add(topPanel, BorderLayout.NORTH);
mainPanel.add(jspView, BorderLayout.CENTER);
JPanel bottomPanel = new JPanel(new BorderLayout());
coveragePanel = new CoveragePanel(this);
bottomPanel.add(coveragePanel, BorderLayout.CENTER);
if(feature_display == null)
{
scrollBar = new JScrollBar(JScrollBar.HORIZONTAL, 1, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
scrollBar.addAdjustmentListener(new AdjustmentListener()
{
public void adjustmentValueChanged(AdjustmentEvent e)
{
repaint();
}
});
bottomPanel.add(scrollBar, BorderLayout.SOUTH);
}
mainPanel.add(bottomPanel, BorderLayout.SOUTH);
coveragePanel.setPreferredSize(new Dimension(900, 100));
coveragePanel.setVisible(false);
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
public void keyPressed(final KeyEvent event)
setZoomLevel((int) (BamView.this.nbasesInView * 1.1));
setZoomLevel((int) (BamView.this.nbasesInView * .9));
addMouseListener(new PopupListener());
public void focusGained(FocusEvent fe){}
public void focusLost(FocusEvent fe){}
JCheckBoxMenuItem checkBoxSingle = new JCheckBoxMenuItem("Single Reads");
checkBoxSingle.addActionListener(new ActionListener()
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public void actionPerformed(ActionEvent e)
{
repaint();
isSingle = !isSingle;
}
});
view.add(checkBoxSingle);
JCheckBoxMenuItem checkBoxSNPs = new JCheckBoxMenuItem("SNPs");
checkBoxSNPs.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if (isSNPs && bases == null)
{
JOptionPane.showMessageDialog(null,
"No reference sequence supplied to identify SNPs.", "SNPs",
JOptionPane.INFORMATION_MESSAGE);
}
isSNPs = !isSNPs;
repaint();
}
});
view.add(checkBoxSNPs);
final JCheckBoxMenuItem checkBoxStackView = new JCheckBoxMenuItem("Stack View");
final JCheckBoxMenuItem checkBoxPairedStackView = new JCheckBoxMenuItem("Paired Stack View");
checkBoxStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
lastend = -1;
isStackView = !isStackView;
if(isStackView)
{
isPairedStackView = !isStackView;
checkBoxPairedStackView.setSelected(!isStackView);
}
checkBoxPairedStackView.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
laststart = -1;
lastend = -1;
isPairedStackView = !isPairedStackView;
if(isPairedStackView)
{
isStackView = !isPairedStackView;
checkBoxStackView.setSelected(!isPairedStackView);
}
repaint();
}
});
view.add(checkBoxPairedStackView);
JCheckBoxMenuItem checkBoxCoverage = new JCheckBoxMenuItem("Coverage");
checkBoxCoverage.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
isCoverage = !isCoverage;
coveragePanel.setVisible(isCoverage);
repaint();
}
});
view.add(checkBoxCoverage);
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view.add(new JSeparator());
JMenu maxHeightMenu = new JMenu("Plot Height");
view.add(maxHeightMenu);
final String hgts[] =
{"500", "800", "1000", "1500", "2500", "5000"};
ButtonGroup bgroup = new ButtonGroup();
for(int i=0; i<hgts.length; i++)
{
final String hgt = hgts[i];
final JCheckBoxMenuItem maxHeightMenuItem = new JCheckBoxMenuItem(hgt);
bgroup.add(maxHeightMenuItem);
maxHeightMenuItem.setSelected(hgts[i].equals(Integer.toString(maxHeight)));
maxHeightMenu.add(maxHeightMenuItem);
maxHeightMenuItem.addActionListener(new ActionListener()
{
public void actionPerformed(ActionEvent e)
{
if(maxHeightMenuItem.isSelected())
maxHeight = Integer.parseInt(hgt);
int start = getBaseAtStartOfView();
setDisplay(start, nbasesInView+start, null);
}
});
}
}
public void setVisible(boolean visible)
{
super.setVisible(visible);
mainPanel.setVisible(visible);
if(feature_display != null)
return feature_display.getForwardBaseAtLeftEdge();
else
return scrollBar.getValue();
}
/**
* Set the panel size based on the number of bases visible
* and repaint.
* @param nbasesInView
*/
if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
this.nbasesInView = (int)(getPreferredSize().getWidth()/pixPerBase);
jspView.getVerticalScrollBar().setValue(0);
jspView.setColumnHeaderView(ruler);
showBaseAlignment = true;
}
else if(jspView != null)
{
jspView.setColumnHeaderView(null);
jspView.getVerticalScrollBar().setValue(
jspView.getVerticalScrollBar().getMaximum());
{
scrollBar.setValues(startValue, nbasesInView, 1,
getMaxBasesInPanel(getSequenceLength()));
scrollBar.setUnitIncrement(nbasesInView/20);
}
* Set the start and end base positions to display.
* @param start
* @param end
* @param event
public void setDisplay(int start,
int end,
DisplayAdjustmentEvent event)
this.startBase = start;
this.endBase = end;
else
{
if(feature_display == null)
pixPerBase = 1000.f/(float)(end-start+1);
else
pixPerBase = feature_display.getWidth()/(float)(end-start+1);
}
if(pixPerBase*1.08f >= ALIGNMENT_PIX_PER_BASE)
if(event == null)
{
this.startBase = -1;
this.endBase = -1;
}
/**
* Return an Artemis entry from a file
* @param entryFileName
* @param entryGroup
* @return
* @throws NoSequenceException
*/
private Entry getEntry(final String entryFileName, final EntryGroup entryGroup)
throws NoSequenceException
{
final Document entry_document = DocumentFactory.makeDocument(entryFileName);
final EntryInformation artemis_entry_information =
Options.getArtemisEntryInformation();
//System.out.println(entryFileName);
final uk.ac.sanger.artemis.io.Entry new_embl_entry =
EntryFileDialog.getEntryFromFile(null, entry_document,
artemis_entry_information,
false);
if(new_embl_entry == null) // the read failed
return null;
Entry entry = null;
try
{
if(entryGroup.getSequenceEntry() != null)
bases = entryGroup.getSequenceEntry().getBases();
else
entry = new Entry(bases,new_embl_entry);
entryGroup.add(entry);
}
catch(OutOfRangeException e)
{
new MessageDialog(null, "read failed: one of the features in " +
entryFileName + " has an out of range " +
"location: " + e.getMessage());
}
return entry;
}
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private boolean isShowScale()
{
return showScale;
}
public void setShowScale(boolean showScale)
{
this.showScale = showScale;
}
public JScrollPane getJspView()
{
return jspView;
}
public void setBases(Bases bases)
{
this.bases = bases;
}
/**
* Remove JScrollPane border
*/
public void removeBorder()
{
Border empty = new EmptyBorder(0,0,0,0);
jspView.setBorder(empty);
}
/**
* Handle a mouse drag event on the drawing canvas.
**/
private void handleCanvasMouseDragOrClick(final MouseEvent event)
if(event.isShiftDown() || event.getButton() == MouseEvent.BUTTON3
|| bases == null)
tjc
committed
if(event.getClickCount() > 1)
{
getSelection().clear();
repaint();
return;
}
tjc
committed
int onmask = MouseEvent.BUTTON1_DOWN_MASK;
int start = (int) ( ( (event.getPoint().getX())/pixPerBase) + getBaseAtStartOfView() );
if (dragStart < 0 && (event.getModifiersEx() & onmask) == onmask)
dragStart = start;
else if((event.getModifiersEx() & onmask) != onmask)
dragStart = -1;
if(start < 1)
start = 1;
if(start > seqLength)
start = seqLength;
if(dragStart < 0)
drag_range = new MarkerRange (bases.getForwardStrand(), start, start);
else
drag_range = new MarkerRange (bases.getForwardStrand(), dragStart, start);
getSelection().setMarkerRange(drag_range);
repaint();
}
}
private Selection getSelection()
{
return selection;
}
public Dimension getPreferredScrollableViewportSize()
{
return getPreferredSize();
}
public int getScrollableBlockIncrement(Rectangle visibleRect,
int orientation, int direction)
{
if (orientation == SwingConstants.HORIZONTAL)
return visibleRect.width - maxUnitIncrement;
else
return visibleRect.height - maxUnitIncrement;
}
public boolean getScrollableTracksViewportHeight()
{
return false;
}
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public boolean getScrollableTracksViewportWidth()
{
return false;
}
public int getScrollableUnitIncrement(Rectangle visibleRect, int orientation,
int direction)
{
//Get the current position.
int currentPosition = 0;
if (orientation == SwingConstants.HORIZONTAL)
currentPosition = visibleRect.x;
else
currentPosition = visibleRect.y;
//Return the number of pixels between currentPosition
//and the nearest tick mark in the indicated direction.
if (direction < 0)
{
int newPosition = currentPosition -
(currentPosition / maxUnitIncrement)
* maxUnitIncrement;
return (newPosition == 0) ? maxUnitIncrement : newPosition;
}
else
{
return ((currentPosition / maxUnitIncrement) + 1)
* maxUnitIncrement
- currentPosition;
}
}
/**
* Artemis event notification
*/
public void displayAdjustmentValueChanged(DisplayAdjustmentEvent event)
{
if(event.getType() == DisplayAdjustmentEvent.SCALE_ADJUST_EVENT)
{
laststart = -1;
lastend = -1;
this.startBase = event.getStart();
this.endBase = event.getEnd();
int width = feature_display.getMaxVisibleBases();
setZoomLevel(width);
repaint();
}
else
{
setDisplay(event.getStart(),
event.getStart()+feature_display.getMaxVisibleBases(), event);
repaint();
}
}
public void selectionChanged(SelectionChangeEvent event)
{
repaint();
public Ruler()
{
super();
setPreferredSize(new Dimension(getPreferredSize().width, 15));
setBackground(Color.white);
setFont(getFont().deriveFont(11.f));
}
public void paintComponent(Graphics g)
{
super.paintComponent(g);
Graphics2D g2 = (Graphics2D)g;
drawBaseScale(g2, start, end, 12);
}
private void drawBaseScale(Graphics2D g2, int start, int end, int ypos)
{
int startMark = (((int)(start/10))*10)+1;
for(int i=startMark; i<end; i+=10)
{
g2.drawString(Integer.toString(i), xpos, ypos);
xpos+=(ALIGNMENT_PIX_PER_BASE/2);
g2.drawLine(xpos, ypos+1, xpos, ypos+5);
}
}
/**
* Popup menu listener
*/
class PopupListener extends MouseAdapter
{
public void mouseClicked(MouseEvent e)
{
BamView.this.requestFocus();
}
public void mousePressed(MouseEvent e)
{
maybeShowPopup(e);
}
public void mouseReleased(MouseEvent e)
{
maybeShowPopup(e);
}
private void maybeShowPopup(MouseEvent e)
{
if(e.isPopupTrigger())
{
if(popup == null)
{
popup = new JPopupMenu();
createViewMenu(popup);
}
popup.show(e.getComponent(),
e.getX(), e.getY());
class PairedRead
{
SAMRecord sam1;
SAMRecord sam2;
}
public static void main(String[] args)
{
String bam = args[0];
for(int i=0;i<args.length; i++)
{
if(args[i].equals("-a"))
bam = args[++i];
else if(args[i].equals("-r"))
reference = args[++i];
else if(args[i].equals("-v"))
nbasesInView = Integer.parseInt(args[++i]);
else if(args[i].equals("-s"))
System.setProperty("samtoolDir", args[++i]);
else if(args[i].startsWith("-h"))
{
System.out.println("-h\t show help");
System.out.println("-a\t BAM/SAM file to display");
System.out.println("-r\t reference file (optional)");
System.out.println("-v\t number of bases to display in the view (optional)");
/*System.out.println("-s\t samtool directory");*/